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Literature summary extracted from

  • Chellamuthu, M.; Kumaresan, K.; Subramanian, S.; Muthumanickam, H.
    Functional analysis of sesame diacylglycerol acyltransferase and phospholipid diacylglycerol acyltransferase genes using in silico and in vitro approaches (2019), Plant Mol. Biol. Rep., 37, 146-156 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene SiDGAT1, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiDGAT gene-transformed mutants, lipid analysis, overview Sesamum indicum
2.3.1.20 gene SiDGAT2, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiDGAT gene-transformed mutants, lipid analysis, overview Sesamum indicum
2.3.1.158 gene SiPDAT, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiPDAT gene-transformed mutants, lipid analysis, overview Sesamum indicum
2.3.1.158 gene SiPDAT, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiPDAT gene-transformed mutants, SiPDAT1-expressing mutants have higher polyunsaturated (C18:1; C18:2) fatty acid content, lipid analysis, overview Sesamum indicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 endoplasmic reticulum membrane
-
 Sesamum indicum 5789
-
2.3.1.20 lipid droplet
-
 Sesamum indicum 5811
-
2.3.1.20 soluble SiDGAT1 A1 and SiDGAT1 A2 do not have any transmembrane domains Sesamum indicum
-
-
2.3.1.158 endoplasmic reticulum membrane
-
 Sesamum indicum 5789
-
2.3.1.158 lipid droplet
-
 Sesamum indicum 5811
-
2.3.1.158 soluble SiPDAT2 does not have any transmembrane domains Sesamum indicum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Sesamum indicum
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol Sesamum indicum
-
 lysophospholipid + triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Sesamum indicum M1E7W9
-
-
2.3.1.20 Sesamum indicum XP_011098009
-
-
2.3.1.158 Sesamum indicum XP_011088820
-
-
2.3.1.158 Sesamum indicum XP_020553631
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 additional information tissue distribution analysis by quantitative real-time PCR Sesamum indicum
-
2.3.1.20 seed highest expression of SiDGAT1 in mature seeds Sesamum indicum
-
2.3.1.20 seed highest expression of SiDGAT2 in mature seeds Sesamum indicum
-
2.3.1.158 flower
-
 Sesamum indicum
-
2.3.1.158 additional information tissue distribution analysis by quantitative real-time PCR. SiPDAT1 expression is highest at flowering stage and developing seeds Sesamum indicum
-
2.3.1.158 additional information tissue distribution analysis by quantitative real-time PCR. SiPDAT2 expression is highest in stem and developing seeds Sesamum indicum
-
2.3.1.158 seed developing Sesamum indicum
-
2.3.1.158 stem
-
 Sesamum indicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Sesamum indicum CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.158 phospholipid + 1,2-diacyl-sn-glycerol
-
 Sesamum indicum lysophospholipid + triacylglycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 ? x * 37484, DGAT2, sequence calculation Sesamum indicum
2.3.1.20 ? x * 61936, DGAT1, sequence calculation Sesamum indicum
2.3.1.158 ? x * 73872, PDAT1, sequence calculation Sesamum indicum
2.3.1.158 ? x * 76594, PDAT2, sequence calculation Sesamum indicum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 DGAT1
-
 Sesamum indicum
2.3.1.20 DGAT2
-
 Sesamum indicum
2.3.1.20 diacylglycerol acyltransferase
-
 Sesamum indicum
2.3.1.20 SiDGAT1
-
 Sesamum indicum
2.3.1.20 SiDGAT2
-
 Sesamum indicum
2.3.1.158 PDAT
-
 Sesamum indicum
2.3.1.158 PDAT2
-
 Sesamum indicum
2.3.1.158 phospholipid:diacylglycerol acyltransferase
-
 Sesamum indicum
2.3.1.158 SiPDAT
-
 Sesamum indicum
2.3.1.158 SiPDAT2
-
 Sesamum indicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.20 Sesamum indicum sequence calculation
-
 8.06
2.3.1.20 Sesamum indicum sequence calculation
-
 9.42
2.3.1.158 Sesamum indicum sequence calculation
-
 8.11
2.3.1.158 Sesamum indicum sequence calculation
-
 8.5

General Information

EC Number General Information Comment Organism
2.3.1.20 metabolism specific role of DGAT (EC 2.3.1.20) and PDAT (EC 2.3.1.158) genes in fatty acid biosynthesis Sesamum indicum
2.3.1.20 physiological function diacylglycerol acyltransferase (DGAT) is an acyl-CoA-dependent enzyme which converts diacylglycerol (DAG) to triacylglycerol (TAG) Sesamum indicum
2.3.1.158 metabolism specific role of DGAT (EC 2.3.1.20) and PDAT (EC 2.3.1.158) genes in fatty acid biosynthesis Sesamum indicum
2.3.1.158 physiological function phospholipid:diacylglycerol acyltransferase (PDAT) is an acyl-CoA-independent pathway enzyme using phosphatidylcholine (PC) as the acyl donor, in which the transfer of an acyl group from the sn-2 position of phosphatidylcholine to the sn-3 position of DAG yields triacylglycerol (TAG) Sesamum indicum