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Literature summary extracted from

  • Wong, H.; Akamatsu, A.; Wang, Q.; Higuchi, M.; Matsuda, T.; Okuda, J.; Kosami, K.; Inada, N.; Kawasaki, T.; Kaneko-Kawano, T.; Nagawa, S.; Tan, L.; Kawano, Y.; Shimamoto, K.
    In vivo monitoring of plant small GTPase activation using a Foerster resonance energy transfer biosensor (2018), Plant Methods, 14, 56 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.2 recombinant expression of Raichu-OsRac1 in rice protoplasts from strain C5924 with high efficiency Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.2 GTP + H2O Oryza sativa Japonica Group
-
 GDP + phosphate
-
 ?
3.6.5.2 GTP + H2O Oryza sativa Japonica Group C5924
-
 GDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.2 Oryza sativa Japonica Group Q9SSX0
-
-
3.6.5.2 Oryza sativa Japonica Group C5924 Q9SSX0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.2 GTP + H2O
-
 Oryza sativa Japonica Group GDP + phosphate
-
 ?
3.6.5.2 GTP + H2O
-
 Oryza sativa Japonica Group C5924 GDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.5.2 OsRac1
-
 Oryza sativa Japonica Group
3.6.5.2 Rac-like GTP-binding protein 1 UniProt Oryza sativa Japonica Group
3.6.5.2 small GTPase
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
3.6.5.2 evolution the enzyme belongs to the Rac/ROP family small GTPases Oryza sativa Japonica Group
3.6.5.2 additional information establishment of a plant version of a Förster resonance energy transfer (FRET) probe called Ras and interacting protein chimeric unit (Raichu) that can successfully monitor activation of the rice small GTPase OsRac1 during various defence responses in cells, protocol for visualizing spatiotemporal activity of plant Rac/ ROP GTPase in living plant cells, transfection of rice protoplasts, method, detailed overview Oryza sativa Japonica Group
3.6.5.2 physiological function small GTPases act as molecular switches that regulate various plant responses such as disease resistance, pollen tube growth, root hair development, cell wall patterning and hormone responses. Small GTPases exhibit both GDP/GTP-binding activity and GTPase activity, and work as molecular switches by cycling between GDP-bound inactive and GTP-bound active forms. Rac/ROP family small GTPases are master regulators, controlling various signalling systems in plants such as those governing pollen tube growth, root hair development, auxin signaling, and disease resistance Oryza sativa Japonica Group