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Literature summary extracted from

  • Liu, D.; Ji, H.; Yang, Z.
    Functional characterization of three novel genes encoding diacylglycerol acyltransferase (DGAT) from oil-rich tubers of Cyperus esculentus (2020), Plant Cell Physiol., 61, 118-129 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 genes CeDGAT1, CeDGAT2a, and CeDGAT2b, functional overexpression of the isozymes in yeast mutant strain H1246, and in Arabidopsis thaliana seeds of TAG1 mutant and wild-type lines via Agrobacterium tumefaciens strain EHA105 transfection method, quantitative RT-PCR enzyme expression analysis Cyperus esculentus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information functional overexpression of CeDGAT2b in yeast mutant and Arabidopsis TAG1 mutant and wild-type lines not only enhances TAG contents but also modifies the fatty acid compositions of TAG. The expression of CeDGAT2b rescues the seed phenotype of Arabidopsis thaliana TAG1 mutant ABX45 Cyperus esculentus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 membrane
-
 Cyperus esculentus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Cyperus esculentus
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Cyperus esculentus
-
 var. sativus
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 leaf highest expression level for CeDGAT1 Cyperus esculentus
-
2.3.1.20 additional information spatiotemporal expression analysis of the isozymes in different tissues of Cyperus esculentus, distinct expression patterns of the CeDGAT isozyme genes are observed in the different tissues, maybe also reflecting their potential role in triacylglycerol (TAG) biosynthesis Cyperus esculentus
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2.3.1.20 root highest expression level for CeDGAT1 Cyperus esculentus
-
2.3.1.20 tuber highest expression level for CeDGAT2b, lowest for CeDGAT2a Cyperus esculentus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Cyperus esculentus CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 additional information isozyme CeDGAT2b from Cyperus esculentus might have a preference for unsaturated fatty acids such as oleic acid as substrates for the production of triacylglycerol (TAG) in tubers Cyperus esculentus ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 CeDGAT1
-
 Cyperus esculentus
2.3.1.20 CeDGAT2a
-
 Cyperus esculentus
2.3.1.20 CeDGAT2b
-
 Cyperus esculentus

General Information

EC Number General Information Comment Organism
2.3.1.20 metabolism the isozyme of DGAT in Cyperus esculentus are involved in the triacylglycerol (TAG) biosynthesis Cyperus esculentus