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Literature summary extracted from

  • Bulman, S.; Richter, F.; Marschollek, S.; Benade, F.; Juelke, S.; Ludwig-Mueller, J.
    Arabidopsis thaliana expressing PbBSMT, a gene encoding a SABATH-type methyltransferase from the plant pathogenic protist Plasmodiophora brassicae, show leaf chlorosis and altered host susceptibility (2019), Plant Biol., 21 Suppl 1, 120-130 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.273 gene BSMT, cloning in Escherichia coli, Arabidopsis thaliana plants are transformed using the floral dip method via Agrobacterium tumefaciens AGL1, recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leading to chlorosis and altered host susceptibility, induced transgenic plants are not able to support large galls and have a brownish appearance of some clubs. The methylester of SA (MeSA) is transported from clubbed Arabidopsis roots to leaves, as shown using heavy isotope-labelled MeSA, and is emitted only from leaves of infected plants, semi- and quantitative RT-PCR expression analysis Plasmodiophora brassicae
2.1.1.274 gene BSMT, cloning in Escherichia coli, Arabidopsis thaliana plants are transformed using the floral dip method via Agrobacterium tumefaciens AGL1, recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leading to chlorosis and altered host susceptibility, induced transgenic plants are not able to support large galls and have a brownish appearance of some clubs. The methylester of SA (MeSA) is transported from clubbed Arabidopsis roots to leaves, as shown using heavy isotope-labelled MeSA, and is emitted only from leaves of infected plants, semi- and quantitative RT-PCR expression analysis Plasmodiophora brassicae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.273 S-adenosyl-L-methionine + benzoate Plasmodiophora brassicae
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + salicylate Plasmodiophora brassicae
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate Plasmodiophora brassicae
-
 methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate Plasmodiophora brassicae
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.273 Plasmodiophora brassicae R4I7S9
-
-
2.1.1.274 Plasmodiophora brassicae R4I7S9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.273 S-adenosyl-L-methionine + anthranilate
-
 Plasmodiophora brassicae methyl anthranilate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoate
-
 Plasmodiophora brassicae methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.273 S-adenosyl-L-methionine + salicylate
-
 Plasmodiophora brassicae methyl salicylate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + anthranilate
-
 Plasmodiophora brassicae methyl anthranilate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + benzoate
-
 Plasmodiophora brassicae methyl benzoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylate
-
 Plasmodiophora brassicae methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.273 benzoate-salicylate methyltransferase
-
 Plasmodiophora brassicae
2.1.1.273 benzoic acid/salicylic acid methyltransferase UniProt Plasmodiophora brassicae
2.1.1.273 BSMT
-
 Plasmodiophora brassicae
2.1.1.273 More see also EC 2.1.1.274 Plasmodiophora brassicae
2.1.1.273 PbBSMT methyltransferase
-
 Plasmodiophora brassicae
2.1.1.274 benzoate-salicylate methyltransferase
-
 Plasmodiophora brassicae
2.1.1.274 benzoic acid/salicylic acid methyltransferase UniProt Plasmodiophora brassicae
2.1.1.274 BSMT
-
 Plasmodiophora brassicae
2.1.1.274 More see also EC 2.1.1.273 Plasmodiophora brassicae
2.1.1.274 PbBSMT methyltransferase
-
 Plasmodiophora brassicae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.273 S-adenosyl-L-methionine
-
 Plasmodiophora brassicae
2.1.1.274 S-adenosyl-L-methionine
-
 Plasmodiophora brassicae

General Information

EC Number General Information Comment Organism
2.1.1.273 malfunction recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leads to chlorosis and altered host susceptibility. Transcription of PbBSMT is associated with: (1) strong leaf phenotypes from anthocyanin accumulation and chlorosis followed by browning, (2) increased plant susceptibility after infection with Plasmodiophora brassicae that is manifested as more yellow leaves and reduced growth of upper plant parts, and (3) induced transgenic plants are not able to support large galls and had a brownish appearance of some clubs. Microarray data indicate that chlorophyll loss is accompanied by reduced transcription of genes involved in photosynthesis, while genes encoding glucose metabolism, mitochondrial functions and cell wall synthesis are upregulated. Phenotype overview Plasmodiophora brassicae
2.1.1.273 physiological function the plant pathogenic protist Plasmodiophora brassicae causes clubroot disease of Brassicaceae. The biotrophic organism can downregulate plant defence responses via its salicylic acid methyltransferase. The enzyme is involved in attenuation of host defence responses in the roots by metabolising a plant defence signal. Role for the methylation of salicylic acid in attenuating plant defence response in infected roots as a strategy for intracellular parasitism. Salicylic acid (SA) is a plant defence hormone that acts as a prominent signal in response to biotrophic pathogens Plasmodiophora brassicae
2.1.1.274 malfunction recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leads to chlorosis and altered host susceptibility. Transcription of PbBSMT is associated with: (1) strong leaf phenotypes from anthocyanin accumulation and chlorosis followed by browning, (2) increased plant susceptibility after infection with Plasmodiophora brassicae that is manifested as more yellow leaves and reduced growth of upper plant parts, and (3) induced transgenic plants are not able to support large galls and had a brownish appearance of some clubs. Microarray data indicate that chlorophyll loss is accompanied by reduced transcription of genes involved in photosynthesis, while genes encoding glucose metabolism, mitochondrial functions and cell wall synthesis are upregulated. Phenotype overview Plasmodiophora brassicae
2.1.1.274 physiological function the plant pathogenic protist Plasmodiophora brassicae causes clubroot disease of Brassicaceae. The biotrophic organism can downregulate plant defence responses via its salicylic acid methyltransferase. The enzyme is involved in attenuation of host defence responses in the roots by metabolising a plant defence signal. Role for the methylation of salicylic acid in attenuating plant defence response in infected roots as a strategy for intracellular parasitism. Salicylic acid (SA) is a plant defence hormone that acts as a prominent signal in response to biotrophic pathogens Plasmodiophora brassicae