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Literature summary extracted from

  • Ma, B.; Liu, X.; Lu, Y.; Ma, X.; Wu, X.; Wang, X.; Jia, M.; Su, P.; Tong, Y.; Guan, H.; Jiang, Z.; Gao, J.; Huang, L.; Gao, W.
    A specific UDP-glucosyltransferase catalyzes the formation of triptophenolide glucoside from Tripterygium wilfordii Hook. f. (2019), Phytochemistry, 166, 112062 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.B79 medicine product triptophenolide 14-O-beta-D-glucopyranoside exhibits significant inhibitory effects on U87-MG, U251, C6, MCF-7, HeLa, K562, and RBL-2H3 cells Tripterygium wilfordii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.B79 molecular docking of substrates. Residue H30 exhibits a hydrogen bond interaction with triptophenolide. Residues W369, A370, Q372, N391, S392, E395, and W396 interact with uridine diphosphate glucose and exhibit hydrogen bond interactions with uridine diphosphate glucose Tripterygium wilfordii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.B79 additional information site-directed mutagenesis of residues His30, Asp132, Phe134, Thr154, Ala370, Leu376, Gly382, His387, Glu395 and Gln412, none of the 11 mutants can catalyze the glycosylation of triptophenolide Tripterygium wilfordii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.B79 0.0127
-
 triptophenolide pH 7.5, 30°C Tripterygium wilfordii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.B79 additional information divalent cations are not required nor improve the activity Tripterygium wilfordii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.B79 Tripterygium wilfordii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.B79 UDP-beta-D-glucose + 4-methylumbelliferone
-
 Tripterygium wilfordii UDP + 4-methylumbelliferyl-beta-D-glucopyranoside
-
 ?
2.4.1.B79 UDP-beta-D-glucose + liquiritigenin
-
 Tripterygium wilfordii UDP + neoliquiritin
-
 ?
2.4.1.B79 UDP-beta-D-glucose + phloretin
-
 Tripterygium wilfordii UDP + phlorizin
-
 ?
2.4.1.B79 UDP-beta-D-glucose + pinocembrin
-
 Tripterygium wilfordii UDP + pinocembrin 7-O-beta-D-glucopyranoside
-
 ?
2.4.1.B79 UDP-beta-D-glucose + rhapontigenin
-
 Tripterygium wilfordii UDP + rhapontin
-
 ?
2.4.1.B79 UDP-beta-D-glucose + triptophenolide
-
 Tripterygium wilfordii UDP + triptophenolide 14-O-beta-D-glucopyranoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.B79 UGT1
-
 Tripterygium wilfordii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.B79 40
-
-
 Tripterygium wilfordii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.B79 0.07
-
 triptophenolide pH 7.5, 30°C Tripterygium wilfordii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.B79 8
-
-
 Tripterygium wilfordii