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Literature summary extracted from

  • Kontturi, J.; Osama, R.; Deng, X.; Bashandy, H.; Albert, V.A.; Teeri, T.H.
    Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida (2017), Phytochemistry, 134, 38-45 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74 expressed in Escherichia coli DH5alpha cells Gerbera hybrid cultivar

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Gerbera hybrid cultivar A0A1D9C6D7
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 His SpinTrap column chromatography Gerbera hybrid cultivar

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 anther highest expression in tapetal cells of the anther Gerbera hybrid cultivar
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 2 malonyl-CoA + 2 stearoyl-CoA
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 Gerbera hybrid cultivar 6-heptadecyl-4-hydroxy-2H-pyran-2-one + 4-hydroxy-6-(2-oxononadecyl)-2H-pyran-2-one + 2 CoA + 2 CO2
-
 ?
2.3.1.74 malonyl-CoA + acetyl-CoA the reaction is catalyzed by isoform ASCL2 only Gerbera hybrid cultivar 4-hydroxy-6-methyl-2-pyrone + CoA + CO2
-
 ?
2.3.1.74 malonyl-CoA + hexanoyl-CoA
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 Gerbera hybrid cultivar 4-hydroxy-6-pentyl-2H-pyran-2-one + CoA + CO2
-
 ?
2.3.1.74 malonyl-CoA + octanoyl-CoA
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 Gerbera hybrid cultivar 6-heptyl-4-hydroxy-2H-pyran-2-one + CoA + CO2
-
 ?
2.3.1.74 additional information neither isoform GASCL1 nor isoform GASCL2 accept 4-coumaroyl-CoA or benzoyl-CoA as substrates Gerbera hybrid cultivar ?
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Subunits

EC Number Subunits Comment Organism
2.3.1.74 ? x * 43000, SDS-PAGE Gerbera hybrid cultivar

Synonyms

EC Number Synonyms Comment Organism
2.3.1.74 anther-specific chalcone synthase-like enzyme
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 Gerbera hybrid cultivar
2.3.1.74 ASCL1
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 Gerbera hybrid cultivar
2.3.1.74 ASCL2
-
 Gerbera hybrid cultivar

General Information

EC Number General Information Comment Organism
2.3.1.74 metabolism the enzyme participates in sporopollenin biosynthesis and exine formation within the locule Gerbera hybrid cultivar