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Literature summary extracted from

  • Xu, R.X.; Zhao, Y.; Gao, S.; Zhang, Y.Y.; Li, D.D.; Lou, H.X.; Cheng, A.X.
    Functional characterization of a plastidal cation-dependent O-methyltransferase from the liverwort Plagiochasma appendiculatum (2015), Phytochemistry, 118, 33-41 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.104 expressed as GFP fusion protein in Escherichia coli and Nicotiana tabacum Plagiochasma appendiculatum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.104 L110A the mutant shows about 9% of wild type activity with caffeic acid as substrate Plagiochasma appendiculatum
2.1.1.104 L110G the mutant shows about 9% of wild type activity with caffeic acid as substrate Plagiochasma appendiculatum
2.1.1.104 L110S the mutant shows about 9% of wild type activity with caffeic acid as substrate Plagiochasma appendiculatum
2.1.1.104 L110V the mutant shows about 29% of wild type activity with caffeic acid as substrate Plagiochasma appendiculatum
2.1.1.104 L110Y the mutant shows about 77% of wild type activity with caffeic acid as substrate Plagiochasma appendiculatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.104 0.04388
-
 quercetin at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.08382
-
 eriodictyol at pH 7.5 and 37°C Plagiochasma appendiculatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.104 plastid
-
 Plagiochasma appendiculatum 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.104 Mg2+ required for activity Plagiochasma appendiculatum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.104 Plagiochasma appendiculatum A0A0U2YR01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.104 nickel column chromatography Plagiochasma appendiculatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.104 0.00271
-
 with coniferyl alcohol as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.00443
-
 with esculetin as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.00569
-
 with coniferaldehyde as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.00705
-
 with caffeoyl aldehyde as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.00897
-
 with caffeoyl-CoA as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.01607
-
 with caffeoyl alcohol as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.01923
-
 with ferulic acid as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.02016
-
 with caffeic acid as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.04714
-
 with eriodictyol as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.05666
-
 with luteolin as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.12782
-
 with quercetin as substrate, at pH 7.5 and 37°C Plagiochasma appendiculatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.104 additional information no activity with kaempferol and dihydrokaempferol Plagiochasma appendiculatum ?
-
-
2.1.1.104 S-adenosyl-L-methionine + caffeic acid
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + ferulic acid
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl alcohol
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + coniferyl alcohol
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl aldehyde
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + coniferaldehyde
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + coniferaldehyde
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + sinapaldehyde
-
 ?
2.1.1.104 S-adenosyl-L-methionine + coniferyl alcohol
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + sinapyl alcohol
-
 ?
2.1.1.104 S-adenosyl-L-methionine + eriodictyol
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + homoeriodictyol + hesperetin
-
 ?
2.1.1.104 S-adenosyl-L-methionine + esculetin
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + scopoletin
-
 ?
2.1.1.104 S-adenosyl-L-methionine + ferulic acid
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + sinapic acid
-
 ?
2.1.1.104 S-adenosyl-L-methionine + luteolin
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + chrysoeriol
-
 ?
2.1.1.104 S-adenosyl-L-methionine + quercetin best substrate Plagiochasma appendiculatum S-adenosyl-L-homocysteine + isorhamnetin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.104 ? x * 33710, calculated from amino acid sequence Plagiochasma appendiculatum
2.1.1.104 ? x * 54000, His-tagged enzyme, SDS-PAGE Plagiochasma appendiculatum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.104 CCoAOMT
-
 Plagiochasma appendiculatum
2.1.1.104 OMT1
-
 Plagiochasma appendiculatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.104 0.067
-
 eriodictyol at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 0.156
-
 quercetin at pH 7.5 and 37°C Plagiochasma appendiculatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.104 Plagiochasma appendiculatum calculated from amino acid sequence
-
 5.4

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.104 0.794
-
 eriodictyol at pH 7.5 and 37°C Plagiochasma appendiculatum
2.1.1.104 3.552
-
 quercetin at pH 7.5 and 37°C Plagiochasma appendiculatum