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Literature summary extracted from

  • Haubrich, B.; Collins, E.; Howard, A.; Wang, Q.; Snell, W.; Miller, M.; Thomas, C.; Pleasant, S.; Nes, W.
    Characterization, mutagenesis and mechanistic analysis of an ancient algal sterol C24-methyltransferase Implications for understanding sterol evolution in the green lineage (2015), Phytochemistry, 113, 64-72 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.41 expression in Escherichia coli Chlamydomonas reinhardtii
2.1.1.142 expression in Escherichia coli Chlamydomonas reinhardtii
2.1.1.143 expression in Escherichia coli Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.41 0.018
-
24(28)-methylene cycloartenol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.41 0.02
-
obtusifoliol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.41 0.022
-
24(28)-methylene lophenol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.41 0.024
-
lanosterol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.41 0.025
-
Cycloartenol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.142 0.018
-
24(28)-methylene cycloartenol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.142 0.02
-
obtusifoliol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.142 0.022
-
24(28)-methylene lophenol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.142 0.024
-
lanosterol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.142 0.025
-
Cycloartenol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.143 0.018
-
24(28)-methylene cycloartenol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.143 0.02
-
obtusifoliol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.143 0.022
-
24(28)-methylene lophenol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.143 0.024
-
lanosterol pH 7.5, 30°C Chlamydomonas reinhardtii
2.1.1.143 0.025
-
Cycloartenol pH 7.5, 30°C Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.41 Chlamydomonas reinhardtii A8IJ34
-
-
2.1.1.142 Chlamydomonas reinhardtii A8IJ34
-
-
2.1.1.143 Chlamydomonas reinhardtii A8IJ34
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.41 additional information enzyme catalyzes both the reactions of EC 2.1.1.142, cycloartenol 24-C-methyltransferase and EC 2.1.1.143, 24-methylenesterol C-methyltransferase. SMT1 catalyzes a sterol methylation pathway by the algal Delta25(27)-olefin route, where methylation proceeds by a conserved SN2 reaction and deprotonation proceeds from the pro-Z methyl group on lanosterol corresponding to C27 Chlamydomonas reinhardtii ?
-
-
2.1.1.41 S-adenosyl-L-methionine + 24(28)-methylene cycloartenol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24(28)-ethylidene cycloartenol
-
?
2.1.1.41 S-adenosyl-L-methionine + 24(28)-methylene lophenol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24(28)-ethylidene lophenol
-
?
2.1.1.41 S-adenosyl-L-methionine + cycloartenol reaction of EC 2.1.1.142 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + cyclolaudenol + 24(28)-methylene cycloartanol
-
?
2.1.1.41 S-adenosyl-L-methionine + lanosterol reaction of EC 2.1.1.142 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24beta-methyl lanosta-8,25(27)-enol + 24(28)-methylene lanosterol
-
?
2.1.1.41 S-adenosyl-L-methionine + obtusifoliol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + ?
-
?
2.1.1.41 S-adenosyl-L-methionine + zymosterol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + fecosterol
-
?
2.1.1.142 additional information enzyme catalyzes both the reactions of EC 2.1.1.142, cycloartenol 24-C-methyltransferase and EC 2.1.1.143, 24-methylenesterol C-methyltransferase. SMT1 catalyzes a sterol methylation pathway by the algal Delta25(27)-olefin route, where methylation proceeds by a conserved SN2 reaction and deprotonation proceeds from the pro-Z methyl group on lanosterol corresponding to C27 Chlamydomonas reinhardtii ?
-
-
2.1.1.142 S-adenosyl-L-methionine + 24(28)-methylene cycloartenol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24(28)-ethylidene cycloartenol
-
?
2.1.1.142 S-adenosyl-L-methionine + 24(28)-methylene lophenol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24(28)-ethylidene lophenol
-
?
2.1.1.142 S-adenosyl-L-methionine + cycloartenol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + cyclolaudenol + 24(28)-methylene cycloartanol
-
?
2.1.1.142 S-adenosyl-L-methionine + lanosterol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24beta-methyl lanosta-8,25(27)-enol + 24(28)-methylene lanosterol
-
?
2.1.1.142 S-adenosyl-L-methionine + obtusifoliol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + ?
-
?
2.1.1.142 S-adenosyl-L-methionine + zymosterol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + fecosterol
-
?
2.1.1.143 additional information enzyme catalyzes both the reactions of EC 2.1.1.142, cycloartenol 24-C-methyltransferase and EC 2.1.1.143, 24-methylenesterol C-methyltransferase. SMT1 catalyzes a sterol methylation pathway by the algal Delta25(27)-olefin route, where methylation proceeds by a conserved SN2 reaction and deprotonation proceeds from the pro-Z methyl group on lanosterol corresponding to C27 Chlamydomonas reinhardtii ?
-
-
2.1.1.143 S-adenosyl-L-methionine + 24(28)-methylene cycloartenol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24(28)-ethylidene cycloartenol
-
?
2.1.1.143 S-adenosyl-L-methionine + 24(28)-methylene lophenol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24(28)-ethylidene lophenol
-
?
2.1.1.143 S-adenosyl-L-methionine + cycloartenol reaction of EC 2.1.1.142 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + cyclolaudenol + 24(28)-methylene cycloartanol
-
?
2.1.1.143 S-adenosyl-L-methionine + lanosterol reaction of EC 2.1.1.142 Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + 24beta-methyl lanosta-8,25(27)-enol + 24(28)-methylene lanosterol
-
?
2.1.1.143 S-adenosyl-L-methionine + obtusifoliol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + ?
-
?
2.1.1.143 S-adenosyl-L-methionine + zymosterol
-
Chlamydomonas reinhardtii S-adenosyl-L-homocysteine + fecosterol
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.41 SMT1
-
Chlamydomonas reinhardtii
2.1.1.142 SMT1
-
Chlamydomonas reinhardtii
2.1.1.143 SMT1
-
Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.41 30 35
-
Chlamydomonas reinhardtii
2.1.1.142 30 35
-
Chlamydomonas reinhardtii
2.1.1.143 30 35
-
Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.41 7 8
-
Chlamydomonas reinhardtii
2.1.1.142 7 8
-
Chlamydomonas reinhardtii
2.1.1.143 7 8
-
Chlamydomonas reinhardtii