EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.99.7 | gene acdS, recombinant expression of His6-tagged ACCD in Escherichia coli strain BL21(DE3) | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.99.7 | 1-amino-2,2-difluorocyclopropane-1-carboxylic acid | rac-DFACC, chemical synthesis, overview. Mechanism of decomposition and inhibition of 1-aminocyclopropane-1-carboxylic acid deaminase, and Michaelis-Menten kinetics. DFACC is unstable under near-physiological conditions where it primarily decomposes via specific-base catalysis to 3-fluoro-2-oxobut-3-enoic acid. Different decomposotion mechanisms are described. Upon incubation with ACC deaminase, DFACC is a slow-dissociating inhibitor of ACC deaminase with submicromolar affinity | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.99.7 | 5-nitroanthranilate + H2O | Escherichia coli | - |
5-nitrosalicylate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.99.7 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.99.7 | recombinant His6-tagged ACCD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.99.7 | 5-nitroanthranilate + H2O | - |
Escherichia coli | 5-nitrosalicylate + NH3 | - |
? | |
3.5.99.7 | additional information | 1H NMR spectroscopy for determination of reaction products and metabolites | Escherichia coli | ? | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.99.7 | ACC deaminase | - |
Escherichia coli |
3.5.99.7 | ACCD | - |
Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.99.7 | 7 | - |
assay at | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.99.7 | additional information | - |
additional information | inhibition kinetics, overview | Escherichia coli |