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Literature summary extracted from

  • Ma, B.; Ma, J.; Liu, D.; Guo, L.; Chen, H.; Ding, J.; Liu, W.; Zhang, H.
    Biochemical and structural characterization of a DNA N6-adenine methyltransferase from Helicobacter pylori (2016), Oncotarget, 7, 40965-40977 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli Helicobacter pylori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.72 apoenzyme, hanging drop vapor diffusion method, using 1.0 M Bis-Tris, pH 9.0, 1.4 M ammonium tartrate. Enzyme in complex with S-adenosyl-L-methionine, hanging drop vapor diffusion method, using 1.0 M Bis-Tris pH 6.0, 14% (w/v) PEG 2000, 0.2 M lithium sulfate Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Helicobacter pylori the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Helicobacter pylori 26695 the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Helicobacter pylori
-
-
-
2.1.1.72 Helicobacter pylori 26695
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 Ni-NTA affinity column chromatography, Mono S column chromatography, and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' Helicobacter pylori S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' Helicobacter pylori 26695 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 dimer gel filtration Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 DNA N6-adenine methyltransferase
-
 Helicobacter pylori
2.1.1.72 M1.HpyAVI
-
 Helicobacter pylori
2.1.1.72 N6-adenine MTase
-
 Helicobacter pylori