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Literature summary extracted from

  • Li, K.; Li, L.; Xu, Y.
    Crystal structure determination of a chimeric FabF by XRD (2017), Nucl. Sci. Tech., 28, 123 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.179 chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells Streptomyces albus
2.3.1.179 chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells Streptomyces platensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.179 sitting drop vapor diffusion method, using either 0.1 M sodium chloride, 0.1 M bicine pH 9.0, 20% (w/v) PEG MME 550 or 0.1 M HEPES pH 7.5, 12% (w/v) PEG 3350 or 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M ammonium sulfate Streptomyces albus
2.3.1.179 sitting drop vapor diffusion method, using either 0.1 M sodium chloride, 0.1 M bicine pH 9.0, 20% (w/v) PEG MME 550 or 0.1 M HEPES pH 7.5, 12% (w/v) PEG 3350 or 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350, 0.2 M ammonium sulfate Streptomyces platensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.179 additional information not inhibited by platensimycin Streptomyces albus
2.3.1.179 additional information not inhibited by platensimycin Streptomyces platensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Streptomyces albus
-
 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Streptomyces platensis
-
 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Streptomyces albus J1074
-
 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.179 Streptomyces albus
-
-
-
2.3.1.179 Streptomyces albus J1074
-
-
-
2.3.1.179 Streptomyces platensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.179 Ni-NTA Sepharose column chromatography and Superdex S200 gel filtration Streptomyces albus
2.3.1.179 Ni-NTA Sepharose column chromatography and Superdex S200 gel filtration Streptomyces platensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Streptomyces albus a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Streptomyces platensis a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.179 a (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Streptomyces albus J1074 a (Z)-3-oxooctadeca-11-enoyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.179 ? x * 50000, chimeric protein js200-FabF, SDS-PAGE Streptomyces albus
2.3.1.179 ? x * 50000, chimeric protein js200-FabF, SDS-PAGE Streptomyces platensis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.179 beta-ketoacyl-acyl-carrier-protein synthase II
-
 Streptomyces albus
2.3.1.179 beta-ketoacyl-acyl-carrier-protein synthase II
-
 Streptomyces platensis
2.3.1.179 FabF
-
 Streptomyces albus
2.3.1.179 FabF
-
 Streptomyces platensis
2.3.1.179 KAS II
-
 Streptomyces albus
2.3.1.179 KAS II
-
 Streptomyces platensis