EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.198 | phylogenetic analysis, recombinant expression of eYFP-tagged GPAT3 in Nicotiana benthamiana leaves via transfection by Agrobacterium tumefaciens strain GV3101, functional overexpression under control of the CaMV35S promoter in and complementation of Arabidopsis thaliana mutant gpat6-2 | Physcomitrium patens |
2.3.1.198 | phylogenetic analysis, recombinant expression of eYFP-tagged GPAT4 in Nicotiana benthamiana leaves via transfection by Agrobacterium tumefaciens strain GV3101, functional overexpression under control of the CaMV35S promoter in and complementation of Arabidopsis thaliana mutant gpat6-2 | Physcomitrium patens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.198 | additional information | targeted disruption of PpGPAT2, DELTAppgpat2 phenotype, overview | Physcomitrium patens |
2.3.1.198 | additional information | targeted mutagenesis of PpGPAT4, DELTAppgpat4 phenotype, overview | Physcomitrium patens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.198 | endoplasmic reticulum | - |
Physcomitrium patens | 5783 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.198 | acyl-CoA + sn-glycerol 3-phosphate | Physcomitrium patens | - |
CoA + 2-acyl-sn-glycerol 3-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.198 | Physcomitrium patens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.198 | gametophore | - |
Physcomitrium patens | - |
2.3.1.198 | phylloid | - |
Physcomitrium patens | - |
2.3.1.198 | plant cuticle | - |
Physcomitrium patens | - |
2.3.1.198 | protonema | - |
Physcomitrium patens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.198 | acyl-CoA + sn-glycerol 3-phosphate | - |
Physcomitrium patens | CoA + 2-acyl-sn-glycerol 3-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.198 | glycerol-3-phosphate acyltransferase | - |
Physcomitrium patens |
2.3.1.198 | PpGPAT2 | - |
Physcomitrium patens |
2.3.1.198 | PpGPAT4 | - |
Physcomitrium patens |
2.3.1.198 | sn-2 acyltransferase | - |
Physcomitrium patens |
2.3.1.198 | sn-2 GPAT | - |
Physcomitrium patens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.198 | evolution | occurrence of land-plant-specific glycerol-3-phosphate acyltransferases is essential for cuticle formation and gametophore development in Physcomitrella patens, sn-2 GPATs harboring both domains by gene recombination are critical to cuticle formation of early land plants. Phylogenetic analysis | Physcomitrium patens |
2.3.1.198 | malfunction | targeted disruption of PpGPAT2 delays growth and gametophore development. Disruption of PpGPAT2 causes increased susceptibility to osmotic and salt stresses in Physcomitrella patens | Physcomitrium patens |
2.3.1.198 | physiological function | glycerol-3-phosphate acyltransferases is essential for cuticle formation and gametophore development in Physcomitrella patens. Glycerol-3-phosphate acyltransferase (GPAT) harboring bifunctional sn-2 acyltransferase/phosphatase activity produces 2-monoacylglycerol, a precursor for cutin synthesis. The cutin layer plays an important role in plant organ formation by providing organ boundaries. Floral organ fusion and permeable cuticle phenotypes of Arabidopsis thaliana mutant gpat6-2 petals are rescued to the wild-type by the expression of PpGPAT2 or PpGPAT4 | Physcomitrium patens |