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Literature summary extracted from

  • Fawke, S.; Torode, T.A.; Gogleva, A.; Fich, E.A.; S?rensen, I.; Yunusov, T.; Rose, J.K.C.; Schornack, S.
    Glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato and Nicotiana benthamiana leaf epidermis (2019), New Phytol., 223, 1547-1559 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.198 gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis Solanum lycopersicum
2.3.1.198 gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis Nicotiana benthamiana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.198 additional information gene GPAT6 knockout phenotype, overview Nicotiana benthamiana
2.3.1.198 additional information leaves of 6-wk-old tomato cv. Micro-Tom or gpat6-a mutant plants are subjected to a detached leaf infection assay, gpat6-a tomato fruits have increased cuticle permeability, gpat6-a tomato leaves have fewer stomata and show an increased rate of water loss compared with the wild-type. Mutant gpat6-a leaf epidermal cells possess a thicker cell wall. The gpat6-a leaf transcriptome reflects changes in cuticle and cell wall processes and stomatal patterning. Phenotype, overview Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.198 cell wall
-
Solanum lycopersicum 5618
-
2.3.1.198 cell wall
-
Nicotiana benthamiana 5618
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.198 acyl-CoA + sn-glycerol 3-phosphate Solanum lycopersicum
-
CoA + 2-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.198 acyl-CoA + sn-glycerol 3-phosphate Nicotiana benthamiana
-
CoA + 2-acyl-sn-glycerol 3-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.198 Nicotiana benthamiana
-
-
-
2.3.1.198 Solanum lycopersicum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.198 epidermis
-
Nicotiana benthamiana
-
2.3.1.198 flower
-
Solanum lycopersicum
-
2.3.1.198 flower
-
Nicotiana benthamiana
-
2.3.1.198 fruit
-
Solanum lycopersicum
-
2.3.1.198 fruit
-
Nicotiana benthamiana
-
2.3.1.198 leaf
-
Solanum lycopersicum
-
2.3.1.198 leaf
-
Nicotiana benthamiana
-
2.3.1.198 seed
-
Solanum lycopersicum
-
2.3.1.198 seed
-
Nicotiana benthamiana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.198 acyl-CoA + sn-glycerol 3-phosphate
-
Solanum lycopersicum CoA + 2-acyl-sn-glycerol 3-phosphate
-
?
2.3.1.198 acyl-CoA + sn-glycerol 3-phosphate
-
Nicotiana benthamiana CoA + 2-acyl-sn-glycerol 3-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.198 glycerol-3-phosphate acyltransferase 6
-
Solanum lycopersicum
2.3.1.198 glycerol-3-phosphate acyltransferase 6
-
Nicotiana benthamiana
2.3.1.198 GPAT6
-
Solanum lycopersicum
2.3.1.198 GPAT6
-
Nicotiana benthamiana
2.3.1.198 NbGPAT6a
-
Nicotiana benthamiana
2.3.1.198 SlGPAT6a
-
Solanum lycopersicum

Expression

EC Number Organism Comment Expression
2.3.1.198 Solanum lycopersicum gene GPAT6 expression is induced during leaf infections with Phytophthora infestans strain 88069 and Phytophthora palmivora strain P16830 up
2.3.1.198 Nicotiana benthamiana gene GPAT6 expression is induced during leaf infections with Phytophthora infestans strain 88069 and Phytophthora palmivora strain P16830 up

General Information

EC Number General Information Comment Organism
2.3.1.198 malfunction a loss-of-function mutation in tomato SlGPAT6 results in increased susceptibility of leaves to Phytophthora infection, concomitant with changes in haustoria morphology. Modulation of GPAT6 expression alters the outer wall diameter of leaf epidermal cells. Tomato gpat6-a mutants have an impaired cell wall-cuticle continuum and fewer stomata, but show increased water loss Solanum lycopersicum
2.3.1.198 malfunction silencing of Nicotiana benthamiana NbGPAT6a increases leaf susceptibility to infection by the oomycetes Phytophthora infestans and Phytophthora palmivora, whereas overexpression of NbGPAT6a-GFP renders the leaves more resistant. Knockdown or knockout of GPAT6 renders leaves more susceptible to Phytophthora infection but more resistant to Bortrytis cinerea infection Nicotiana benthamiana
2.3.1.198 metabolism cutin biosynthesis involves the esterification of oxygenated 16- or 18-carbon fatty acids to glycerol through the action of glycerol-3-phosphate acyltransferases (GPAT4, GPAT6 and GPAT8). These enzymes have specificity for the second carbon of the glycerol (sn-2 position) Solanum lycopersicum
2.3.1.198 metabolism cutin biosynthesis involves the esterification of oxygenated 16- or 18-carbon fatty acids to glycerol through the action of glycerol-3-phosphate acyltransferases (GPAT4, GPAT6 and GPAT8). These enzymes have specificity for the second carbon of the glycerol (sn-2 position) Nicotiana benthamiana
2.3.1.198 additional information constitutive expression of NbGPAT6a renders leaves resistant to Phytophthora infection, NbGPAT6-mediated resistance is associated with longer-term leaf development processes. Modulating GPAT6 expression alters the thickness of the outer cell walls of the leaf epidermis Nicotiana benthamiana
2.3.1.198 physiological function glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of Nicotiana benthamiana leaf epidermis. Dual functionality of pathogen-inducible GPAT6 in controlling pathogen entry and cell wall properties affecting dehydration in leaves. Role for GPAT6-generated cutin monomers in influencing epidermal cell properties that are integral to leaf-microbe interactions and in limiting dehydration Nicotiana benthamiana
2.3.1.198 physiological function glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato leaf epidermis. Dual functionality of pathogen-inducible GPAT6 in controlling pathogen entry and cell wall properties affecting dehydration in leaves. Role for GPAT6-generated cutin monomers in influencing epidermal cell properties that are integral to leaf-microbe interactions and in limiting dehydration Solanum lycopersicum