EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.198 | gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis | Solanum lycopersicum |
2.3.1.198 | gene GPAT6, phylogenetic analysis, quantitative reverse transcription PCR expression analysis | Nicotiana benthamiana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.198 | additional information | gene GPAT6 knockout phenotype, overview | Nicotiana benthamiana |
2.3.1.198 | additional information | leaves of 6-wk-old tomato cv. Micro-Tom or gpat6-a mutant plants are subjected to a detached leaf infection assay, gpat6-a tomato fruits have increased cuticle permeability, gpat6-a tomato leaves have fewer stomata and show an increased rate of water loss compared with the wild-type. Mutant gpat6-a leaf epidermal cells possess a thicker cell wall. The gpat6-a leaf transcriptome reflects changes in cuticle and cell wall processes and stomatal patterning. Phenotype, overview | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.198 | cell wall | - |
Solanum lycopersicum | 5618 | - |
2.3.1.198 | cell wall | - |
Nicotiana benthamiana | 5618 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.198 | acyl-CoA + sn-glycerol 3-phosphate | Solanum lycopersicum | - |
CoA + 2-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.198 | acyl-CoA + sn-glycerol 3-phosphate | Nicotiana benthamiana | - |
CoA + 2-acyl-sn-glycerol 3-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.198 | Nicotiana benthamiana | - |
- |
- |
2.3.1.198 | Solanum lycopersicum | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.198 | epidermis | - |
Nicotiana benthamiana | - |
2.3.1.198 | flower | - |
Solanum lycopersicum | - |
2.3.1.198 | flower | - |
Nicotiana benthamiana | - |
2.3.1.198 | fruit | - |
Solanum lycopersicum | - |
2.3.1.198 | fruit | - |
Nicotiana benthamiana | - |
2.3.1.198 | leaf | - |
Solanum lycopersicum | - |
2.3.1.198 | leaf | - |
Nicotiana benthamiana | - |
2.3.1.198 | seed | - |
Solanum lycopersicum | - |
2.3.1.198 | seed | - |
Nicotiana benthamiana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.198 | acyl-CoA + sn-glycerol 3-phosphate | - |
Solanum lycopersicum | CoA + 2-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.198 | acyl-CoA + sn-glycerol 3-phosphate | - |
Nicotiana benthamiana | CoA + 2-acyl-sn-glycerol 3-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.198 | glycerol-3-phosphate acyltransferase 6 | - |
Solanum lycopersicum |
2.3.1.198 | glycerol-3-phosphate acyltransferase 6 | - |
Nicotiana benthamiana |
2.3.1.198 | GPAT6 | - |
Solanum lycopersicum |
2.3.1.198 | GPAT6 | - |
Nicotiana benthamiana |
2.3.1.198 | NbGPAT6a | - |
Nicotiana benthamiana |
2.3.1.198 | SlGPAT6a | - |
Solanum lycopersicum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.3.1.198 | Solanum lycopersicum | gene GPAT6 expression is induced during leaf infections with Phytophthora infestans strain 88069 and Phytophthora palmivora strain P16830 | up |
2.3.1.198 | Nicotiana benthamiana | gene GPAT6 expression is induced during leaf infections with Phytophthora infestans strain 88069 and Phytophthora palmivora strain P16830 | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.198 | malfunction | a loss-of-function mutation in tomato SlGPAT6 results in increased susceptibility of leaves to Phytophthora infection, concomitant with changes in haustoria morphology. Modulation of GPAT6 expression alters the outer wall diameter of leaf epidermal cells. Tomato gpat6-a mutants have an impaired cell wall-cuticle continuum and fewer stomata, but show increased water loss | Solanum lycopersicum |
2.3.1.198 | malfunction | silencing of Nicotiana benthamiana NbGPAT6a increases leaf susceptibility to infection by the oomycetes Phytophthora infestans and Phytophthora palmivora, whereas overexpression of NbGPAT6a-GFP renders the leaves more resistant. Knockdown or knockout of GPAT6 renders leaves more susceptible to Phytophthora infection but more resistant to Bortrytis cinerea infection | Nicotiana benthamiana |
2.3.1.198 | metabolism | cutin biosynthesis involves the esterification of oxygenated 16- or 18-carbon fatty acids to glycerol through the action of glycerol-3-phosphate acyltransferases (GPAT4, GPAT6 and GPAT8). These enzymes have specificity for the second carbon of the glycerol (sn-2 position) | Solanum lycopersicum |
2.3.1.198 | metabolism | cutin biosynthesis involves the esterification of oxygenated 16- or 18-carbon fatty acids to glycerol through the action of glycerol-3-phosphate acyltransferases (GPAT4, GPAT6 and GPAT8). These enzymes have specificity for the second carbon of the glycerol (sn-2 position) | Nicotiana benthamiana |
2.3.1.198 | additional information | constitutive expression of NbGPAT6a renders leaves resistant to Phytophthora infection, NbGPAT6-mediated resistance is associated with longer-term leaf development processes. Modulating GPAT6 expression alters the thickness of the outer cell walls of the leaf epidermis | Nicotiana benthamiana |
2.3.1.198 | physiological function | glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of Nicotiana benthamiana leaf epidermis. Dual functionality of pathogen-inducible GPAT6 in controlling pathogen entry and cell wall properties affecting dehydration in leaves. Role for GPAT6-generated cutin monomers in influencing epidermal cell properties that are integral to leaf-microbe interactions and in limiting dehydration | Nicotiana benthamiana |
2.3.1.198 | physiological function | glycerol-3-phosphate acyltransferase 6 controls filamentous pathogen interactions and cell wall properties of the tomato leaf epidermis. Dual functionality of pathogen-inducible GPAT6 in controlling pathogen entry and cell wall properties affecting dehydration in leaves. Role for GPAT6-generated cutin monomers in influencing epidermal cell properties that are integral to leaf-microbe interactions and in limiting dehydration | Solanum lycopersicum |