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Literature summary extracted from

  • Shinozaki, J.; Kenmoku, H.; Nihei, K.; Masuda, K.; Noji, M.; Konno, K.; Asakawa, Y.; Kazuma, K.
    Cloning and functional analysis of three chalcone synthases from the flowers of safflowers Carthamus tinctorius (2016), Nat. Prod. Commun., 11, 787-790 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74 expressed in Escherichia coli BL21(DE3) cells Carthamus tinctorius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA Carthamus tinctorius
-
 4 CoA + naringenin chalcone + 3 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Carthamus tinctorius A0A1B4XSV2
-
-
2.3.1.74 Carthamus tinctorius A0A1B4XSV4
-
-
2.3.1.74 Carthamus tinctorius A0A1B4XSV6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 MidiTrap G-25 column chromatography Carthamus tinctorius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 flower
-
 Carthamus tinctorius
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Carthamus tinctorius 4 CoA + naringenin chalcone + 3 CO2
-
 ?
2.3.1.74 3 malonyl-CoA + caffeoyl-CoA
-
 Carthamus tinctorius ?
-
 ?
2.3.1.74 3 malonyl-CoA + feruloyl-CoA
-
 Carthamus tinctorius ?
-
 ?
2.3.1.74 3 malonyl-CoA + sinapoyl-CoA
-
 Carthamus tinctorius ?
-
 ?
2.3.1.74 malonyl-CoA + caffeoyl-CoA
-
 Carthamus tinctorius ?
-
 ?
2.3.1.74 malonyl-CoA + feruloyl-CoA
-
 Carthamus tinctorius ?
-
 ?
2.3.1.74 malonyl-CoA + sinapoyl-CoA
-
 Carthamus tinctorius ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.74 ? x * 42800, isoform CHS3, calculated from amino acid sequence Carthamus tinctorius
2.3.1.74 ? x * 43400, isoform CHS1, calculated from amino acid sequence Carthamus tinctorius
2.3.1.74 ? x * 43600, isoform CHS2, calculated from amino acid sequence Carthamus tinctorius

Synonyms

EC Number Synonyms Comment Organism
2.3.1.74 Chs1
-
 Carthamus tinctorius
2.3.1.74 Chs2
-
 Carthamus tinctorius
2.3.1.74 CHS3
-
 Carthamus tinctorius