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Literature summary extracted from

  • Cho, J.; Lee, C.; Zhao, J.; Young, H.; Zhou, P.
    Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis (2016), Nat. Microbiol., 1, 16154 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.54 expressed in Escherichia coli BL21(DE3) cells Haemophilus influenzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.54 in complex with lipid X, using 100 mM NaCl, 10 mM HEPES, 35 mM sodium acetate pH 4.1, 2.2% (w/v) PEG 4000, 0.3 mM sulfonyl piperazine, 0.7% (v/v) DMSO, 22% (v/v) glycerol, and about 3-4 mM n-decyl-beta-D-thiomaltopyranoside Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.54 UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine + H2O Haemophilus influenzae
-
 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl 1-phosphate + UMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.54 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.54 Ni-NTA column chromatography and Sephacryl S-200 gel filtration Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.54 UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine + H2O
-
 Haemophilus influenzae 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl 1-phosphate + UMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.54 LpxH
-
 Haemophilus influenzae
3.6.1.54 UDP-diacylglucosamine pyrophosphohydrolase
-
 Haemophilus influenzae