EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.167 | synthesis | mutant enzyme G38R/F301V with a catalytic efficiency approximately six times higher than that of the wild-type is combined with a beta-xylosidase to obtain an in vitro one-pot conversion of 7-beta-xylosyl-10-deacetyltaxol to Taxol yielding 0.64 mg/mlx02taxol in 50 ml at 15 h. This approach represents a promising environmentally friendly alternative for Taxol production from an abundant analogue | Taxus cuspidata |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.167 | expression in Escherichia coli | Taxus cuspidata |
2.3.1.167 | expression in Escherichia coli | Taxus canadensis |
2.3.1.167 | expression in Escherichia coli | Taxus x media |
2.3.1.167 | expression in Escherichia coli | Taxus brevifolia |
2.3.1.167 | expression in Escherichia coli | Taxus wallichiana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.167 | F160A | mutation negatively affects activity | Taxus cuspidata |
2.3.1.167 | F301A | activity against 10-deacetyltaxol is 1.6times more active than wild-type activity, activitys against 10-deacetylbaccatin III is degraded | Taxus cuspidata |
2.3.1.167 | F301V | mutant enzyme is 2.85times more active against 10-deacetyltaxol than the wild-type enzyme | Taxus cuspidata |
2.3.1.167 | F400A | mutation negatively affects activity | Taxus cuspidata |
2.3.1.167 | F44A | activity against 10-deacetyltaxol is nearly undetectable | Taxus cuspidata |
2.3.1.167 | G359A | mutation negatively affects activity | Taxus cuspidata |
2.3.1.167 | G361A | no activity against 10-deacetylbaccatin III or 10-deacetyltaxol | Taxus cuspidata |
2.3.1.167 | G38A | activity against 10-deacetyltaxol is 1.45times more active than wild-type activity, activitys against 10-deacetylbaccatin III is slightly decreased | Taxus cuspidata |
2.3.1.167 | G38R | mutant enzyme is 2.19times more active against 10-deacetyltaxol than the wild-type enzyme | Taxus cuspidata |
2.3.1.167 | G38R/F301V | mutant enzyme is 3.7times more active against 10-deacetyltaxol than the wild-type enzyme, activity against 10-deacetylbaccatin III is 69% as compared to wild-type enzyme | Taxus cuspidata |
2.3.1.167 | H162A | no activity against 10-deacetylbaccatin III or 10-deacetyltaxol | Taxus cuspidata |
2.3.1.167 | I164A | no activity against 10-deacetylbaccatin III or 10-deacetyltaxol | Taxus cuspidata |
2.3.1.167 | P37A | mutation negatively affects activity | Taxus cuspidata |
2.3.1.167 | R363A | no activity against 10-deacetylbaccatin III or 10-deacetyltaxol | Taxus cuspidata |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.167 | 0.178 | - |
10-deacetyltaxol | mutant enzyme F301V, pH 5.5, 37.5°C | Taxus cuspidata | |
2.3.1.167 | 0.368 | - |
10-deacetyltaxol | mutant enzyme G38R, pH 5.5, 37.5°C | Taxus cuspidata | |
2.3.1.167 | 0.424 | - |
10-deacetyltaxol | mutant enzyme G38R/F301V, pH 5.5, 37.5°C | Taxus cuspidata | |
2.3.1.167 | 0.546 | - |
10-deacetyltaxol | wild-type enzyme, pH 5.5, 37.5° | Taxus cuspidata |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.167 | Taxus brevifolia | B5KRG4 | - |
- |
2.3.1.167 | Taxus canadensis | B5KRF5 | - |
- |
2.3.1.167 | Taxus cuspidata | Q9M6E2 | - |
- |
2.3.1.167 | Taxus wallichiana | B5KRG1 | - |
- |
2.3.1.167 | Taxus x media | Q6SQJ5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.167 | - |
Taxus cuspidata |
2.3.1.167 | - |
Taxus canadensis |
2.3.1.167 | - |
Taxus x media |
2.3.1.167 | - |
Taxus brevifolia |
2.3.1.167 | - |
Taxus wallichiana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.167 | acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus cuspidata | CoA + baccatin III | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus canadensis | CoA + baccatin III | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus x media | CoA + baccatin III | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus brevifolia | CoA + baccatin III | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetylbaccatin III | - |
Taxus wallichiana | CoA + baccatin III | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetyltaxol | - |
Taxus cuspidata | CoA + paclitaxel | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetyltaxol | - |
Taxus canadensis | CoA + taxol | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetyltaxol | - |
Taxus x media | CoA + taxol | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetyltaxol | - |
Taxus brevifolia | CoA + taxol | - |
? | |
2.3.1.167 | acetyl-CoA + 10-deacetyltaxol | - |
Taxus wallichiana | CoA + taxol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.167 | 10-deacetylbaccatin III-10-beta-O-acetyltransferase | - |
Taxus cuspidata |
2.3.1.167 | 10-deacetylbaccatin III-10-beta-O-acetyltransferase | - |
Taxus canadensis |
2.3.1.167 | 10-deacetylbaccatin III-10-beta-O-acetyltransferase | - |
Taxus x media |
2.3.1.167 | 10-deacetylbaccatin III-10-beta-O-acetyltransferase | - |
Taxus brevifolia |
2.3.1.167 | 10-deacetylbaccatin III-10-beta-O-acetyltransferase | - |
Taxus wallichiana |
2.3.1.167 | DBAT | - |
Taxus cuspidata |
2.3.1.167 | DBAT | - |
Taxus canadensis |
2.3.1.167 | DBAT | - |
Taxus x media |
2.3.1.167 | DBAT | - |
Taxus brevifolia |
2.3.1.167 | DBAT | - |
Taxus wallichiana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.167 | 38 | - |
substrate: 10-deacetyltaxol | Taxus cuspidata |
2.3.1.167 | 40 | - |
substrate: 10-deacetylbaccatin III | Taxus cuspidata |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.167 | 0.000334 | - |
10-deacetyltaxol | wild-type enzyme, pH 5.5, 37.5° | Taxus cuspidata | |
2.3.1.167 | 0.000396 | - |
10-deacetyltaxol | mutant enzyme F301V, pH 5.5, 37.5°C | Taxus cuspidata | |
2.3.1.167 | 0.000815 | - |
10-deacetyltaxol | mutant enzyme G38R, pH 5.5, 37.5°C | Taxus cuspidata | |
2.3.1.167 | 0.001499 | - |
10-deacetyltaxol | mutant enzyme G38R/F301V, pH 5.5, 37.5°C | Taxus cuspidata |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.167 | 5.5 | - |
substrates: 10-deacetylbaccatin III, 10-deacetyltaxol | Taxus cuspidata |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.167 | 0.000611 | - |
10-deacetyltaxol | wild-type enzyme, pH 5.5, 37.5°C | Taxus cuspidata | |
2.3.1.167 | 0.002218 | - |
10-deacetyltaxol | mutant enzyme G38R, pH 5.5, 37.5°C | Taxus cuspidata | |
2.3.1.167 | 0.00222 | - |
10-deacetyltaxol | mutant enzyme F301V, pH 5.5, 37.5°C | Taxus cuspidata | |
2.3.1.167 | 0.003539 | - |
10-deacetyltaxol | mutant enzyme G38R/F301V, pH 5.5, 37.5°C | Taxus cuspidata |