Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, B.J.; Wang, H.; Gong, T.; Chen, J.J.; Chen, T.J.; Yang, J.L.; Zhu, P.
    Improving 10-deacetylbaccatin III-10-beta-O-acetyltransferase catalytic fitness for Taxol production (2017), Nat. Commun., 8, 15544 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.167 synthesis mutant enzyme G38R/F301V with a catalytic efficiency approximately six times higher than that of the wild-type is combined with a beta-xylosidase to obtain an in vitro one-pot conversion of 7-beta-xylosyl-10-deacetyltaxol to Taxol yielding 0.64 mg/mlx02taxol in 50 ml at 15 h. This approach represents a promising environmentally friendly alternative for Taxol production from an abundant analogue Taxus cuspidata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.167 expression in Escherichia coli Taxus cuspidata
2.3.1.167 expression in Escherichia coli Taxus canadensis
2.3.1.167 expression in Escherichia coli Taxus x media
2.3.1.167 expression in Escherichia coli Taxus brevifolia
2.3.1.167 expression in Escherichia coli Taxus wallichiana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.167 F160A mutation negatively affects activity Taxus cuspidata
2.3.1.167 F301A activity against 10-deacetyltaxol is 1.6times more active than wild-type activity, activitys against 10-deacetylbaccatin III is degraded Taxus cuspidata
2.3.1.167 F301V mutant enzyme is 2.85times more active against 10-deacetyltaxol than the wild-type enzyme Taxus cuspidata
2.3.1.167 F400A mutation negatively affects activity Taxus cuspidata
2.3.1.167 F44A activity against 10-deacetyltaxol is nearly undetectable Taxus cuspidata
2.3.1.167 G359A mutation negatively affects activity Taxus cuspidata
2.3.1.167 G361A no activity against 10-deacetylbaccatin III or 10-deacetyltaxol Taxus cuspidata
2.3.1.167 G38A activity against 10-deacetyltaxol is 1.45times more active than wild-type activity, activitys against 10-deacetylbaccatin III is slightly decreased Taxus cuspidata
2.3.1.167 G38R mutant enzyme is 2.19times more active against 10-deacetyltaxol than the wild-type enzyme Taxus cuspidata
2.3.1.167 G38R/F301V mutant enzyme is 3.7times more active against 10-deacetyltaxol than the wild-type enzyme, activity against 10-deacetylbaccatin III is 69% as compared to wild-type enzyme Taxus cuspidata
2.3.1.167 H162A no activity against 10-deacetylbaccatin III or 10-deacetyltaxol Taxus cuspidata
2.3.1.167 I164A no activity against 10-deacetylbaccatin III or 10-deacetyltaxol Taxus cuspidata
2.3.1.167 P37A mutation negatively affects activity Taxus cuspidata
2.3.1.167 R363A no activity against 10-deacetylbaccatin III or 10-deacetyltaxol Taxus cuspidata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.167 0.178
-
10-deacetyltaxol mutant enzyme F301V, pH 5.5, 37.5°C Taxus cuspidata
2.3.1.167 0.368
-
10-deacetyltaxol mutant enzyme G38R, pH 5.5, 37.5°C Taxus cuspidata
2.3.1.167 0.424
-
10-deacetyltaxol mutant enzyme G38R/F301V, pH 5.5, 37.5°C Taxus cuspidata
2.3.1.167 0.546
-
10-deacetyltaxol wild-type enzyme, pH 5.5, 37.5° Taxus cuspidata

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.167 Taxus brevifolia B5KRG4
-
-
2.3.1.167 Taxus canadensis B5KRF5
-
-
2.3.1.167 Taxus cuspidata Q9M6E2
-
-
2.3.1.167 Taxus wallichiana B5KRG1
-
-
2.3.1.167 Taxus x media Q6SQJ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.167
-
Taxus cuspidata
2.3.1.167
-
Taxus canadensis
2.3.1.167
-
Taxus x media
2.3.1.167
-
Taxus brevifolia
2.3.1.167
-
Taxus wallichiana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
Taxus cuspidata CoA + baccatin III
-
?
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
Taxus canadensis CoA + baccatin III
-
?
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
Taxus x media CoA + baccatin III
-
?
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
Taxus brevifolia CoA + baccatin III
-
?
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
Taxus wallichiana CoA + baccatin III
-
?
2.3.1.167 acetyl-CoA + 10-deacetyltaxol
-
Taxus cuspidata CoA + paclitaxel
-
?
2.3.1.167 acetyl-CoA + 10-deacetyltaxol
-
Taxus canadensis CoA + taxol
-
?
2.3.1.167 acetyl-CoA + 10-deacetyltaxol
-
Taxus x media CoA + taxol
-
?
2.3.1.167 acetyl-CoA + 10-deacetyltaxol
-
Taxus brevifolia CoA + taxol
-
?
2.3.1.167 acetyl-CoA + 10-deacetyltaxol
-
Taxus wallichiana CoA + taxol
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.167 10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
Taxus cuspidata
2.3.1.167 10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
Taxus canadensis
2.3.1.167 10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
Taxus x media
2.3.1.167 10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
Taxus brevifolia
2.3.1.167 10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
Taxus wallichiana
2.3.1.167 DBAT
-
Taxus cuspidata
2.3.1.167 DBAT
-
Taxus canadensis
2.3.1.167 DBAT
-
Taxus x media
2.3.1.167 DBAT
-
Taxus brevifolia
2.3.1.167 DBAT
-
Taxus wallichiana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.167 38
-
substrate: 10-deacetyltaxol Taxus cuspidata
2.3.1.167 40
-
substrate: 10-deacetylbaccatin III Taxus cuspidata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.167 0.000334
-
10-deacetyltaxol wild-type enzyme, pH 5.5, 37.5° Taxus cuspidata
2.3.1.167 0.000396
-
10-deacetyltaxol mutant enzyme F301V, pH 5.5, 37.5°C Taxus cuspidata
2.3.1.167 0.000815
-
10-deacetyltaxol mutant enzyme G38R, pH 5.5, 37.5°C Taxus cuspidata
2.3.1.167 0.001499
-
10-deacetyltaxol mutant enzyme G38R/F301V, pH 5.5, 37.5°C Taxus cuspidata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.167 5.5
-
substrates: 10-deacetylbaccatin III, 10-deacetyltaxol Taxus cuspidata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.167 0.000611
-
10-deacetyltaxol wild-type enzyme, pH 5.5, 37.5°C Taxus cuspidata
2.3.1.167 0.002218
-
10-deacetyltaxol mutant enzyme G38R, pH 5.5, 37.5°C Taxus cuspidata
2.3.1.167 0.00222
-
10-deacetyltaxol mutant enzyme F301V, pH 5.5, 37.5°C Taxus cuspidata
2.3.1.167 0.003539
-
10-deacetyltaxol mutant enzyme G38R/F301V, pH 5.5, 37.5°C Taxus cuspidata