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Literature summary extracted from

  • Gupta, Y.K.; Chan, S.H.; Xu, S.Y.; Aggarwal, A.K.
    Structural basis of asymmetric DNA methylation and ATP-triggered long-range diffusion by EcoP15I (2015), Nat. Commun., 6, 7363 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli HMS174 (DE3) and ER2566 cells Caulobacter vibrioides
2.1.1.72 expressed in Escherichia coli NEB cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.72 in complex with duplex DNA and AMP, hanging drop vapor diffusion method, using 10% (w/v) PEG 5000 monomethyl ether, 0.1 M HEPES pH 7.5, 0.2M potassium acetate and 15 mM MnCl2 at 20°C Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 D347A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 H317A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
2.1.1.72 H336A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
2.1.1.72 K267A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 K318A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 N330A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
2.1.1.72 Q324A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 R268A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 R272A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
2.1.1.72 R302A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
2.1.1.72 R350A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides
2.1.1.72 R354A the mutant shows wild type-like methylation activity Caulobacter vibrioides
2.1.1.72 S315A the mutant shows a severely decreased methylation activity (to less than 10%) compared to the wild type enzyme Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + adenine in DNA Escherichia coli the enzyme specifically binds double-stranded DNA S-adenosyl-L-homocysteine + DNA containing N6-methyladenine
-
?
2.1.1.72 S-adenosyl-L-methionine + mRNA Caulobacter vibrioides
-
S-adenosyl-L-homocysteine + mRNA containing N6-methyladenine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Caulobacter vibrioides
-
-
-
2.1.1.72 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 ceramic hydroxylapatite column chromatography and cation exchange column chromatography Escherichia coli
2.1.1.72 Ni-NTA column chromatography Caulobacter vibrioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 additional information the enzyme does not specifically bind to DNA containing GANTC sequences Caulobacter vibrioides ?
-
-
2.1.1.72 S-adenosyl-L-methionine + adenine in DNA the enzyme specifically binds double-stranded DNA Escherichia coli S-adenosyl-L-homocysteine + DNA containing N6-methyladenine
-
?
2.1.1.72 S-adenosyl-L-methionine + mRNA
-
Caulobacter vibrioides S-adenosyl-L-homocysteine + mRNA containing N6-methyladenine
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 ? x * 39000, SDS-PAGE Caulobacter vibrioides

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 CcrM
-
Caulobacter vibrioides
2.1.1.72 DNA MTase
-
Caulobacter vibrioides
2.1.1.72 DNA-(adenine N6)-methyltransferase
-
Caulobacter vibrioides
2.1.1.72 EcoP15I
-
Escherichia coli