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Literature summary extracted from

  • Horton, J.R.; Woodcock, C.B.; Opot, S.B.; Reich, N.O.; Zhang, X.; Cheng, X.
    The cell cycle-regulated DNA adenine methyltransferase CcrM opens a bubble at its DNA recognition site (2019), Nat. Commun., 10, 4600 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in Escherichia coli BL21(DE3) cells Caulobacter vibrioides

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.72 in complex with double-stranded DNA containing the recognition sequence GANTC, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.8, with 10% (w/v) polyethylene glycol 8000 and 3% (w/v) polyethylene glycol 6000 Caulobacter vibrioides

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.72 S315A/H317A/N330A/R350A the mutant shows about 90% reduced methylation activity on double stranded DNA but no effect on S-adenosyl-L-methionione binding Caulobacter vibrioides
2.1.1.72 W332A inactive Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Caulobacter vibrioides the enzyme methylates the adenine of hemimethylated GANTC after replication S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Caulobacter vibrioides P0CAW2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72 HisTrap nickel affinity column chromatography Caulobacter vibrioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme methylates the adenine of hemimethylated GANTC after replication Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 dimer
-
 Caulobacter vibrioides

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 CcrM
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 Caulobacter vibrioides
2.1.1.72 cell cycle-regulated DNA methyltransferase
-
 Caulobacter vibrioides
2.1.1.72 DNA adenine methyltransferase
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 Caulobacter vibrioides