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Literature summary extracted from
- Biochemical and biophysical characterisation of haloalkane dehalogenases DmrA and DmrB in Mycobacterium strain JS60 and their role in growth on haloalkanes (2015), Mol. Microbiol., 97, 439-453 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | expressed in Escherichia coli BL21(DE3) cells | Mycolicibacterium rhodesiae |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | hanging-drop vapour diffusion method, crystal structure of selenomethionyl-DmrA is determined to 1.7 A resolution | Mycolicibacterium rhodesiae |
| 3.8.1.5 | selenomethionyl-labelled enzyme DmrA, hanging drop vapor diffusion method, using 0.1 M bis-Tris propane, pH 6.5, 0.2 M KSCN, 16% (w/v) PEG 3350 | Mycolicibacterium rhodesiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | 1.9 | - |
4-bromobutyronitrile | at pH 8.0 and 25°C | Mycolicibacterium rhodesiae |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 35000 | - |
gel filtration | Mycolicibacterium rhodesiae |
| 3.8.1.5 | 150000 | - |
gel filtration | Mycolicibacterium rhodesiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.5 | Mycolicibacterium rhodesiae | G4I2J6 | - |
- |
| 3.8.1.5 | Mycolicibacterium rhodesiae | G4I5P8 | - |
- |
| 3.8.1.5 | Mycolicibacterium rhodesiae JS60 | G4I2J6 | - |
- |
| 3.8.1.5 | Mycolicibacterium rhodesiae JS60 | G4I5P8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.8.1.5 | - |
Mycolicibacterium rhodesiae |
| 3.8.1.5 | Ni-NTA agarose column chromatography and Superdex 75 gel filtration | Mycolicibacterium rhodesiae |
| 3.8.1.5 | Ni-NTA agarose column chromatography, UNO Q6 anion exchange column chromatography and Superdex 75 gel filtration | Mycolicibacterium rhodesiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.5 | 1,2-dibromoethane + H2O | - |
Mycolicibacterium rhodesiae | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB | Mycolicibacterium rhodesiae | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | about 90% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | strong dehalogenase activity | Mycolicibacterium rhodesiae | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | about 90% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae JS60 | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | - |
Mycolicibacterium rhodesiae JS60 | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromoethane + H2O | about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB | Mycolicibacterium rhodesiae JS60 | 2-bromoethanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dibromohexane + H2O | - |
Mycolicibacterium rhodesiae | 2-bromohexanol + bromide | - |
? | |
| 3.8.1.5 | 1,2-dichloroethane + H2O | low activity | Mycolicibacterium rhodesiae | 2-chloroethanol + chloride | - |
? | |
| 3.8.1.5 | 1,2-dichloroethane + H2O | low activity | Mycolicibacterium rhodesiae JS60 | 2-chloroethanol + chloride | - |
? | |
| 3.8.1.5 | 1,6-dibromohexane + H2O | - |
Mycolicibacterium rhodesiae | 6-bromohexanol + bromide | - |
? | |
| 3.8.1.5 | 1,6-dibromohexane + H2O | about 25% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae | 6-bromohexanol + bromide | - |
? | |
| 3.8.1.5 | 1,6-dibromohexane + H2O | about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB | Mycolicibacterium rhodesiae | 6-bromohexanol + bromide | - |
? | |
| 3.8.1.5 | 1,6-dibromohexane + H2O | about 45% of the specific activity compared to 1-bromohexane, enzyme form DmrB | Mycolicibacterium rhodesiae JS60 | 6-bromohexanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromobutane + H2O | - |
Mycolicibacterium rhodesiae | 1-butanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromobutane + H2O | strong dehalogenase activity | Mycolicibacterium rhodesiae | 1-butanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromohexane + H2O | - |
Mycolicibacterium rhodesiae | 1-hexanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromohexane + H2O | about 35% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae | 1-hexanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromohexane + H2O | clearly preferred substrate | Mycolicibacterium rhodesiae | 1-hexanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromohexane + H2O | enzyme form DmrB | Mycolicibacterium rhodesiae | 1-hexanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopentane + H2O | - |
Mycolicibacterium rhodesiae | 1-pentanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopentane + H2O | about 15% of the specific activity compared to 1-bromohexane, enzyme form DmrB | Mycolicibacterium rhodesiae | 1-pentanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopentane + H2O | about 85% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae | 1-pentanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopentane + H2O | strong dehalogenase activity | Mycolicibacterium rhodesiae | 1-pentanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopropane + H2O | - |
Mycolicibacterium rhodesiae | 1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1-bromopropane + H2O | about 60% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae | 1-propanol + bromide | - |
? | |
| 3.8.1.5 | 1-iodobutane + H2O | - |
Mycolicibacterium rhodesiae | 1-butanol + iodide | - |
? | |
| 3.8.1.5 | 1-iodobutane + H2O | about 50% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae | 1-butanol + iodide | - |
? | |
| 3.8.1.5 | 1-iodopropane + H2O | - |
Mycolicibacterium rhodesiae | 1-propanol + iodide | - |
? | |
| 3.8.1.5 | 1-iodopropane + H2O | about 50% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae | 1-propanol + iodide | - |
? | |
| 3.8.1.5 | 4-bromobutyronitrile + H2O | - |
Mycolicibacterium rhodesiae | 4-hydroxybutyronitrile + bromide | - |
? | |
| 3.8.1.5 | 4-bromobutyronitrile + H2O | strong dehalogenase activity | Mycolicibacterium rhodesiae | 4-hydroxybutyronitrile + bromide | - |
? | |
| 3.8.1.5 | 4-bromobutyronitrile + H2O | about 20% of the specific activity compared to 1-bromohexane, enzyme form DmrB | Mycolicibacterium rhodesiae | ? + bromide | - |
? | |
| 3.8.1.5 | 4-bromobutyronitrile + H2O | about 85% of the specific activity compared to 1-bromobutane, enzyme form DmrA | Mycolicibacterium rhodesiae | ? + bromide | - |
? | |
| 3.8.1.5 | bromocyclohexane + H2O | low activity | Mycolicibacterium rhodesiae | cyclohexanol + bromide | - |
? | |
| 3.8.1.5 | additional information | active against C2-C6 haloalkanes, with a preference for brominated linear substrates | Mycolicibacterium rhodesiae | ? | - |
- |
|
| 3.8.1.5 | additional information | active against C2-C6 haloalkanes, with a preference for brominated linear substrates, enzyme form DmrA | Mycolicibacterium rhodesiae | ? | - |
- |
|
| 3.8.1.5 | additional information | enzyme DmrA is active against mono- and dihalogenated alkanes up to six carbons in length and favours brominated species over iodinated or chlorinated ones and linear substrates over cyclic ones | Mycolicibacterium rhodesiae | ? | - |
- |
|
| 3.8.1.5 | additional information | no activity with chloroform | Mycolicibacterium rhodesiae | ? | - |
- |
|
| 3.8.1.5 | additional information | no activity with chloroform and 1,1,2-trichloroethane | Mycolicibacterium rhodesiae | ? | - |
- |
|
| 3.8.1.5 | additional information | active against C2-C6 haloalkanes, with a preference for brominated linear substrates, enzyme form DmrA | Mycolicibacterium rhodesiae JS60 | ? | - |
- |
|
| 3.8.1.5 | additional information | enzyme DmrA is active against mono- and dihalogenated alkanes up to six carbons in length and favours brominated species over iodinated or chlorinated ones and linear substrates over cyclic ones | Mycolicibacterium rhodesiae JS60 | ? | - |
- |
|
| 3.8.1.5 | additional information | no activity with chloroform and 1,1,2-trichloroethane | Mycolicibacterium rhodesiae JS60 | ? | - |
- |
|
| 3.8.1.5 | additional information | active against C2-C6 haloalkanes, with a preference for brominated linear substrates | Mycolicibacterium rhodesiae JS60 | ? | - |
- |
|
| 3.8.1.5 | additional information | no activity with chloroform | Mycolicibacterium rhodesiae JS60 | ? | - |
- |
|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | homotetramer | 4 * 37100, multi-angle laser-light scattering analysis | Mycolicibacterium rhodesiae |
| 3.8.1.5 | monomer | 1 * 35500, multi-angle laser-light scattering analysis | Mycolicibacterium rhodesiae |
| 3.8.1.5 | monomer | DmrA | Mycolicibacterium rhodesiae |
| 3.8.1.5 | tetramer | DmrB | Mycolicibacterium rhodesiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.8.1.5 | DmrA | - |
Mycolicibacterium rhodesiae |
| 3.8.1.5 | DmrB | - |
Mycolicibacterium rhodesiae |
| 3.8.1.5 | HLD | - |
Mycolicibacterium rhodesiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 38 | - |
- |
Mycolicibacterium rhodesiae |
| 3.8.1.5 | 38 | - |
substrate: 1-bromobutane | Mycolicibacterium rhodesiae |
| 3.8.1.5 | 38 | - |
substrate: 1-bromohexane | Mycolicibacterium rhodesiae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.5 | 3.1 | - |
4-bromobutyronitrile | at pH 8.0 and 25°C | Mycolicibacterium rhodesiae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.5 | 8 | - |
- |
Mycolicibacterium rhodesiae |
| 3.8.1.5 | 8 | - |
substrate: 1-bromohexane | Mycolicibacterium rhodesiae |
| 3.8.1.5 | 8.5 | - |
- |
Mycolicibacterium rhodesiae |
| 3.8.1.5 | 8.5 | - |
substrate: 1-bromobutane | Mycolicibacterium rhodesiae |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.8.1.5 | Mycolicibacterium rhodesiae | DmrA shows significantly higher levels of upregulation in response to haloalkanes than DmrB | up |
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