Literature summary extracted from
Chung, J.; Chen, T.; Missiakas, D.
Transfer of electrons across the cytoplasmic membrane by DsbD, a membrane protein involved in thiol-disulphide exchange and protein folding in the bacterial periplasm (2000), Mol. Microbiol., 35, 1099-1109 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.8.4.16 |
expressed in Escherichia coli BL21(DE3) cells |
Escherichia coli |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.8.4.16 |
C103A |
inactive |
Escherichia coli |
1.8.4.16 |
C109A |
inactive |
Escherichia coli |
1.8.4.16 |
C163A |
inactive |
Escherichia coli |
1.8.4.16 |
C282A |
inactive |
Escherichia coli |
1.8.4.16 |
C464A |
inactive |
Escherichia coli |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.8.4.16 |
cytoplasmic membrane |
- |
Escherichia coli |
- |
- |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.8.4.16 |
a [protein] carrying a disulfide bond + thioredoxin |
Escherichia coli |
overall reaction |
a [protein] with reduced L-cysteine residues + thioredoxin disulfide |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.8.4.16 |
Escherichia coli |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.8.4.16 |
Ni-NTA column chromatography and Mono-Q Sepharose column chromatography |
Escherichia coli |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.8.4.16 |
a [protein] carrying a disulfide bond + thioredoxin |
overall reaction |
Escherichia coli |
a [protein] with reduced L-cysteine residues + thioredoxin disulfide |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.8.4.16 |
? |
x * 60000, SDS-PAGE |
Escherichia coli |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.8.4.16 |
DsbD |
- |
Escherichia coli |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.8.4.16 |
metabolism |
cytoplasmic electrons donated by thioredoxin are transferred into the periplasm via the enzyme |
Escherichia coli |