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Literature summary extracted from

  • Li, B.; Maezato, Y.; Kim, S.H.; Kurihara, S.; Liang, J.; Michael, A.J.
    Polyamine-independent growth and biofilm formation, and functional spermidine/spermine N-acetyltransferases in Staphylococcus aureus and Enterococcus faecalis (2019), Mol. Microbiol., 111, 159-175 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.57 expressed in Escherichia coli BL21(DE3) cells Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.57 0.045
-
 spermine at pH 7.5 and 30°C Enterococcus faecalis
2.3.1.57 0.295
-
 spermine at pH 7.5 and 30°C Staphylococcus aureus
2.3.1.57 0.769
-
 spermidine at pH 7.5 and 30°C Enterococcus faecalis
2.3.1.57 1.33
-
 spermidine at pH 7.5 and 30°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.57 acetyl-CoA + norspermidine Staphylococcus aureus
-
 CoA + N1-acetylnorspermidine
-
 ?
2.3.1.57 acetyl-CoA + norspermidine Staphylococcus aureus USA300
-
 CoA + N1-acetylnorspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermidine Staphylococcus aureus
-
 CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermidine Staphylococcus aureus USA300
-
 CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermine Staphylococcus aureus preferred substrate CoA + N1-acetylspermine
-
 ?
2.3.1.57 acetyl-CoA + spermine Staphylococcus aureus USA300 preferred substrate CoA + N1-acetylspermine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Enterococcus faecalis
-
-
-
2.3.1.57 Staphylococcus aureus A0A0H2XGJ0
-
-
2.3.1.57 Staphylococcus aureus USA300 A0A0H2XGJ0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.57 Hi-Trap column chromatography Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 acetyl-CoA + N1-acetylspermine
-
 Enterococcus faecalis CoA + N1,N12-diacetylspermine
-
 ?
2.3.1.57 acetyl-CoA + norspermidine
-
 Enterococcus faecalis CoA + N1-acetylnorspermidine
-
 ?
2.3.1.57 acetyl-CoA + norspermidine
-
 Staphylococcus aureus CoA + N1-acetylnorspermidine
-
 ?
2.3.1.57 acetyl-CoA + norspermidine
-
 Staphylococcus aureus USA300 CoA + N1-acetylnorspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermidine
-
 Enterococcus faecalis CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermidine
-
 Staphylococcus aureus CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermidine
-
 Staphylococcus aureus USA300 CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermine preferred substrate Enterococcus faecalis CoA + N1-acetylspermine
-
 ?
2.3.1.57 acetyl-CoA + spermine preferred substrate Staphylococcus aureus CoA + N1-acetylspermine
-
 ?
2.3.1.57 acetyl-CoA + spermine preferred substrate Staphylococcus aureus USA300 CoA + N1-acetylspermine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.57 bltD
-
 Enterococcus faecalis
2.3.1.57 SpeG
-
 Staphylococcus aureus
2.3.1.57 spermidine/spermine N-acetyltransferase
-
 Enterococcus faecalis
2.3.1.57 spermidine/spermine N-acetyltransferase
-
 Staphylococcus aureus
2.3.1.57 SSAT
-
 Enterococcus faecalis
2.3.1.57 SSAT
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.57 1.58
-
 spermidine at pH 7.5 and 30°C Enterococcus faecalis
2.3.1.57 2.48
-
 spermidine at pH 7.5 and 30°C Staphylococcus aureus
2.3.1.57 3.52
-
 spermine at pH 7.5 and 30°C Staphylococcus aureus
2.3.1.57 9.21
-
 spermine at pH 7.5 and 30°C Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.57 1.8
-
 spermidine at pH 7.5 and 30°C Staphylococcus aureus
2.3.1.57 2
-
 spermidine at pH 7.5 and 30°C Enterococcus faecalis
2.3.1.57 12
-
 spermine at pH 7.5 and 30°C Staphylococcus aureus
2.3.1.57 200
-
 spermine at pH 7.5 and 30°C Enterococcus faecalis