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Literature summary extracted from

  • Lee, W.C.; Jeong, M.C.; Lee, Y.; Kwak, C.; Lee, J.Y.; Kim, Y.
    Structure and substrate specificity of beta-ketoacyl-acyl carrier protein synthase III from Acinetobacter baumannii (2018), Mol. Microbiol., 108, 567-577 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 expressed in Escherichia coli BL21(DE3) cells Acinetobacter baumannii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.180 apoenzyme or mutant enzymes C155A, C264A and C155A/C264A in complex with octanoyl-CoA, using PEG3350 as a precipitant and Tris HCl buffer (pH 7.0-8.0) and 0.1 M calcium acetate Acinetobacter baumannii

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.180 F56W the mutation does not show a drastic change in reactivity towards octanoyl-CoA even though the band of the variant is reduced approximately by 40% compared to the wild type Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein] Acinetobacter baumannii
-
 an acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Acinetobacter baumannii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.180 Sepharose column chromatography Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein]
-
 Acinetobacter baumannii an acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 octanoyl-CoA + malonyl-[acyl-carrier protein]
-
 Acinetobacter baumannii 3-oxo-decanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Acinetobacter baumannii
2.3.1.180 KAS III
-
 Acinetobacter baumannii