Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, N.; Yu, M.; Zhao, Y.; Cheng, J.; An, K.; Zhang, X.H.
    PfmA, a novel quorum-quenching N-acylhomoserine lactone acylase from Pseudoalteromonas flavipulchra (2017), Microbiology, 163, 1389-1398 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.97 gene pfmA, sequence comparisons, cloning of PfmA and recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Pseudoalteromonas flavipulchra

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.97 H278S site-directed mutagenesis, the mutant shows 80% reduced activity compared to wild-type Pseudoalteromonas flavipulchra
3.5.1.97 L279S site-directed mutagenesis, the mutant shows 74% reduced activity compared to wild-type Pseudoalteromonas flavipulchra

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.97 Mn2+ about 10% inhibition at 1 mM Pseudoalteromonas flavipulchra
3.5.1.97 Ni2+ about 10% inhibition at 1 mM Pseudoalteromonas flavipulchra
3.5.1.97 SDS complete inhibition at 1 mM Pseudoalteromonas flavipulchra
3.5.1.97 Urea 20% inhibition at 1 mM Pseudoalteromonas flavipulchra
3.5.1.97 Zn2+ about 10% inhibition at 1 mM Pseudoalteromonas flavipulchra

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.97 intracellular PfmA lacks an N-terminal signal peptide and no activity is found in the cell medium Pseudoalteromonas flavipulchra 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.97 Co2+ increases the enzyme activity by 8.5% at 1 mM Pseudoalteromonas flavipulchra
3.5.1.97 additional information Na+, K+, Ca2+, Fe3+, and Cu2+ show no or poor effects on AHL acylase activity at 1 mM Pseudoalteromonas flavipulchra

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.97 91000
-
 about, sequence calculation Pseudoalteromonas flavipulchra

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.97 additional information Pseudoalteromonas flavipulchra the marine bacterium Pseudoalteromonas flavipulchra strain JG1 can degrade N-acylhomoserine lactones (AHLs). PfmA can degrade ampicillin but not penicillin G. The concentration of AHLs in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1 is reduced when 0.01 mg/ml PfmA is present ?
-
-
3.5.1.97 additional information Pseudoalteromonas flavipulchra JG1 the marine bacterium Pseudoalteromonas flavipulchra strain JG1 can degrade N-acylhomoserine lactones (AHLs). PfmA can degrade ampicillin but not penicillin G. The concentration of AHLs in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1 is reduced when 0.01 mg/ml PfmA is present ?
-
-
3.5.1.97 N-3-oxo-decanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra
-
 L-homoserine lactone + 3-oxodecanoate
-
 ?
3.5.1.97 N-3-oxo-dodecanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra
-
 L-homoserine lactone + 3-oxododecanoate
-
 ?
3.5.1.97 N-3-oxo-tetradecanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra
-
 L-homoserine lactone + 3-oxotetradecanoate
-
 ?
3.5.1.97 N-decanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra
-
 L-homoserine lactone + decanoate
-
 ?
3.5.1.97 N-decanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra JG1
-
 L-homoserine lactone + decanoate
-
 ?
3.5.1.97 N-dodecanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra
-
 L-homoserine lactone + dodecanoate
-
 ?
3.5.1.97 N-dodecanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra JG1
-
 L-homoserine lactone + dodecanoate
-
 ?
3.5.1.97 N-tetradecanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra
-
 L-homoserine lactone + tetradecanoate
-
 ?
3.5.1.97 N-tetradecanoyl-L-homoserine lactone + H2O Pseudoalteromonas flavipulchra JG1
-
 L-homoserine lactone + tetradecanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.97 Pseudoalteromonas flavipulchra A0A223AMX9
-
-
3.5.1.97 Pseudoalteromonas flavipulchra JG1 A0A223AMX9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.97 proteolytic modification post-translational modification is performed prior to PfmA becoming an active enzyme Pseudoalteromonas flavipulchra

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.97 recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudoalteromonas flavipulchra

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.97 additional information the marine bacterium Pseudoalteromonas flavipulchra strain JG1 can degrade N-acylhomoserine lactones (AHLs). PfmA can degrade ampicillin but not penicillin G. The concentration of AHLs in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1 is reduced when 0.01 mg/ml PfmA is present Pseudoalteromonas flavipulchra ?
-
-
3.5.1.97 additional information enzyme AHL acylase PfmA can degrade N-acylhomoserine lactones (AHLs) with acyl chains longer than 10 carbons. PfmA can degrade ampicillin but not penicillin G. No activity with N-hexanoyl-L-homoserine lactone, N-3-oxo-hexanoyl-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and N-3-oxo-octanoyl-L-homoserine lactone Pseudoalteromonas flavipulchra ?
-
-
3.5.1.97 additional information the marine bacterium Pseudoalteromonas flavipulchra strain JG1 can degrade N-acylhomoserine lactones (AHLs). PfmA can degrade ampicillin but not penicillin G. The concentration of AHLs in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1 is reduced when 0.01 mg/ml PfmA is present Pseudoalteromonas flavipulchra JG1 ?
-
-
3.5.1.97 additional information enzyme AHL acylase PfmA can degrade N-acylhomoserine lactones (AHLs) with acyl chains longer than 10 carbons. PfmA can degrade ampicillin but not penicillin G. No activity with N-hexanoyl-L-homoserine lactone, N-3-oxo-hexanoyl-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and N-3-oxo-octanoyl-L-homoserine lactone Pseudoalteromonas flavipulchra JG1 ?
-
-
3.5.1.97 N-3-oxo-decanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra L-homoserine lactone + 3-oxodecanoate
-
 ?
3.5.1.97 N-3-oxo-dodecanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra L-homoserine lactone + 3-oxododecanoate
-
 ?
3.5.1.97 N-3-oxo-tetradecanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra L-homoserine lactone + 3-oxotetradecanoate
-
 ?
3.5.1.97 N-decanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra L-homoserine lactone + decanoate
-
 ?
3.5.1.97 N-decanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra JG1 L-homoserine lactone + decanoate
-
 ?
3.5.1.97 N-dodecanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra L-homoserine lactone + dodecanoate
-
 ?
3.5.1.97 N-dodecanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra JG1 L-homoserine lactone + dodecanoate
-
 ?
3.5.1.97 N-tetradecanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra L-homoserine lactone + tetradecanoate
-
 ?
3.5.1.97 N-tetradecanoyl-L-homoserine lactone + H2O
-
 Pseudoalteromonas flavipulchra JG1 L-homoserine lactone + tetradecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.97 heterodimer 1 * 26000, alpha-subunit, + 1 * 68000, beta-subunit, His6-tagged recombinant enzyme, SDS-PAGE Pseudoalteromonas flavipulchra
3.5.1.97 More second-structure analysis of PfmA shows that the protein includes an alpha subunit, a spacer sequence, and a beta subunit. The alpha subunit of PfmA is predicted to have seven alpha helices, two beta folds and three random coils, while the beta subunit is predicted to have 12 alpha helices, 19 beta folds and eight random coils Pseudoalteromonas flavipulchra

Synonyms

EC Number Synonyms Comment Organism
3.5.1.97 FaGL1422
-
 Pseudoalteromonas flavipulchra
3.5.1.97 N-acylhomoserine lactone acylase
-
 Pseudoalteromonas flavipulchra
3.5.1.97 pfmA
-
 Pseudoalteromonas flavipulchra
3.5.1.97 quorum-quenching N-acylhomoserine lactone acylase
-
 Pseudoalteromonas flavipulchra

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.97 30
-
-
 Pseudoalteromonas flavipulchra

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.97 20 80 over 40% of maximal activity at 20-80°C, profile overview Pseudoalteromonas flavipulchra

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.97 20 30 purified recombinant enzyme, completely stable at Pseudoalteromonas flavipulchra
3.5.1.97 40
-
 purified recombinant enzyme, 40% activity remaining Pseudoalteromonas flavipulchra
3.5.1.97 50
-
 purified recombinant enzyme, 30% activity remaining Pseudoalteromonas flavipulchra
3.5.1.97 60
-
 purified recombinant enzyme, 10% activity remaining Pseudoalteromonas flavipulchra
3.5.1.97 70
-
 purified recombinant enzyme, 5% activity remaining Pseudoalteromonas flavipulchra
3.5.1.97 80
-
 purified recombinant enzyme, no activity remaining Pseudoalteromonas flavipulchra

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.97 7
-
-
 Pseudoalteromonas flavipulchra

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.97 4 11 over 50% of maximal activity at pH 5.0-11.0, 40% at pH 4.0, profile overview Pseudoalteromonas flavipulchra

General Information

EC Number General Information Comment Organism
3.5.1.97 evolution enzyme PfmA belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily and shows homology to a member of penicillin amidases, but PfmA can degrade ampicillin but not penicillin G Pseudoalteromonas flavipulchra
3.5.1.97 additional information residue Ser256 in PfmA is the active site residue and residues Arg528, Asn324, His278 and Leu279 also play important roles in the catalytic process. Ser356 is also the first amino acid of the beta-subunit Pseudoalteromonas flavipulchra
3.5.1.97 physiological function enzyme PfmA reduces N-acylhomoserine lactone (AHL) accumulation and the production of virulence factors in Vibrio anguillarum strain VIB72 and Pseudomonas aeruginosa strain PAO1, and attenuates the virulence of Pseudomonas aeruginosa to increase Artemia survival, which suggests that PfmA can be considered as a therapeutic agent to control AHL-mediated pathogenicity. N-acylhomoserine lactone acylase is a quorum-quenching enzyme. Quorum sensing (QS) is a cell-to-cell communication mechanism dependent on cell density, which allows bacterial populations to regulate the expression of genes responsible for a wide range of biological behaviours, such as the production of pigments, biofilm formation, antibiotic production, motility, adhesion, bioluminescence and secretion of virulence factors Pseudoalteromonas flavipulchra