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Literature summary extracted from

  • Tada, T.; Miyoshi-Akiyama, T.; Shimada, K.; Dahal, R.; Mishra, S.; Ohara, H.; Kirikae, T.; Pokhrel, B.
    A novel 6'-N-aminoglycoside acetyltransferase, AAC(6)-Ial, from a clinical isolate of Serratia marcescens (2016), Microb. Drug Resist., 22, 103-108 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.82 expression in Escherichia coli Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.012
-
 sisomicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.023
-
 netilmicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.028
-
 tobramycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.086
-
 amikacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.322
-
 kanamycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.399
-
 arbekacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.404
-
 isepamicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.47
-
 neomycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.582
-
 dibekacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Serratia marcescens V5YUM6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + amikacin
-
 Serratia marcescens CoA + N6'-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + arbekacin
-
 Serratia marcescens CoA + 6'-N-acetylarbekacin
-
 ?
2.3.1.82 acetyl-CoA + dibekacin
-
 Serratia marcescens CoA + N6'-acetyldibekacin
-
 ?
2.3.1.82 acetyl-CoA + isepamicin
-
 Serratia marcescens CoA + 6'-N-acetylisepamicin
-
 ?
2.3.1.82 acetyl-CoA + kanamycin
-
 Serratia marcescens CoA + 6'-N-acetylkanamycin
-
 ?
2.3.1.82 acetyl-CoA + neomycin
-
 Serratia marcescens CoA + 6'-N-acetylneomycin
-
 ?
2.3.1.82 acetyl-CoA + netilmicin
-
 Serratia marcescens CoA + 6'-N-acetylnetilmicin
-
 ?
2.3.1.82 acetyl-CoA + sisomicin
-
 Serratia marcescens CoA + 6'-N-acetylsisomicin
-
 ?
2.3.1.82 acetyl-CoA + tobramycin
-
 Serratia marcescens CoA + 6'-N-acetyltobramycin
-
 ?
2.3.1.82 additional information enzyme acetylates all the aminoglycosides tested, except for apramycin, gentamicin, and lividomycin Serratia marcescens ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.82 AAC(6')-IaI
-
 Serratia marcescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.82 0.2
-
 amikacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.49
-
 sisomicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.67
-
 tobramycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 0.73
-
 netilmicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 7.8
-
 neomycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 10
-
 kanamycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 17
-
 arbekacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 25
-
 isepamicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 85
-
 dibekacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.82 2
-
 amikacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 24
-
 tobramycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 25
-
 neomycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 32
-
 netilmicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 32
-
 kanamycin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 41
-
 sisomicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 48
-
 arbekacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 61
-
 isepamicin pH not specified in the publication, temperature not specified in the publication Serratia marcescens
2.3.1.82 150
-
 dibekacin pH not specified in the publication, temperature not specified in the publication Serratia marcescens