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Literature summary extracted from

  • Sonnendecker, C.; Wei, R.; Kurze, E.; Wang, J.; Oeser, T.; Zimmermann, W.
    Efficient extracellular recombinant production and purification of a Bacillus cyclodextrin glucanotransferase in Escherichia coli (2017), Microb. Cell Fact., 16, 87 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Escherichia coli BL21(DE3) cells Bacillus sp. G-825-6

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. G-825-6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 Ni-Sepharose 6 column chromatography Bacillus sp. G-825-6

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 starch + maltose
-
 Bacillus sp. G-825-6 cyclodextrins
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
-
 Bacillus sp. G-825-6
2.4.1.19 cgtS
-
 Bacillus sp. G-825-6
2.4.1.19 cyclodextrin glucanotransferase
-
 Bacillus sp. G-825-6

Expression

EC Number Organism Comment Expression
2.4.1.19 Bacillus sp. G-825-6 the signal peptide DacD enables a 7.3fold increase of activity in the extracellular fraction after induction for 24 h compared to the native enzyme up