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Literature summary extracted from

  • Nguyen, T.; Xu, Y.; Abdel-Hameed, M.; Sorensen, J.L.; Singer, S.D.; Chen, G.
    Characterization of a type-2 diacylglycerol acyltransferase from Haematococcus pluvialis reveals possible allostery of the recombinant enzyme (2020), Lipids, 55, 425-433.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene DGAT2, identified from a transcriptome database, DNA and amino acid sequence determination and analysis of DGAT2 isozymes, DGAT2A, DGAT2B, DGAT2D, and DGAT2E, sequence comparisons and phylogenetic analysis, recombinant expression in TAG-deficient Saccharomyces cerevisiae strain H1246. Triacylglycerol (TAG) is synthesized with expression of HpDGAT2A and HPDGAT2D, but no TAG is detected in cells expressing HpDGAT2B and HpDGAT2E Haematococcus lacustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.20 additional information
-
 additional information Michaelis-Menten or allosteric sigmoidal kinetics Haematococcus lacustris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 membrane isozymes HpDGAT2B and HpDGAT2D contain two transmembrane domains each, while isozymes HpDGAT2A and HpDGAT2E do not Haematococcus lacustris 16020
-
2.3.1.20 microsome
-
 Haematococcus lacustris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+ required Haematococcus lacustris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.20 25275
-
 HpDGAT2A isozyme, sequence calculation Haematococcus lacustris
2.3.1.20 34864
-
 HpDGAT2E isozyme, sequence calculation Haematococcus lacustris
2.3.1.20 37606
-
 HpDGAT2D isozyme, sequence calculation Haematococcus lacustris
2.3.1.20 39912
-
 HpDGAT2B isozyme, sequence calculation Haematococcus lacustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Haematococcus lacustris
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Haematococcus lacustris UTEX 2505
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Haematococcus lacustris A0A699ZU33
-
-
2.3.1.20 Haematococcus lacustris UTEX 2505 A0A699ZU33
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Haematococcus lacustris CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Haematococcus lacustris UTEX 2505 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 additional information the TAG isolated from yeast producing HpDGAT2D contains about 80% of 18:1 and 16:1 and about 20% of 18:0 and 16:0, suggesting thatHpDGAT2D may have a lower preference for 16:0-containing substrate Haematococcus lacustris ?
-
-
2.3.1.20 additional information the TAG isolated from yeast producing HpDGAT2D contains about 80% of 18:1 and 16:1 and about 20% of 18:0 and 16:0, suggesting thatHpDGAT2D may have a lower preference for 16:0-containing substrate Haematococcus lacustris UTEX 2505 ?
-
-
2.3.1.20 oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Haematococcus lacustris CoA + 1,2,3-trioleoyl-sn-glycerol
-
 ?
2.3.1.20 oleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Haematococcus lacustris UTEX 2505 CoA + 1,2,3-trioleoyl-sn-glycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 DGAT
-
 Haematococcus lacustris
2.3.1.20 DGAT2
-
 Haematococcus lacustris
2.3.1.20 diacylglycerol acyltransferase
-
 Haematococcus lacustris
2.3.1.20 HpDGAT2A
-
 Haematococcus lacustris
2.3.1.20 HpDGAT2B
-
 Haematococcus lacustris
2.3.1.20 HpDGAT2D
-
 Haematococcus lacustris
2.3.1.20 HpDGAT2E
-
 Haematococcus lacustris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 30
-
 assay at Haematococcus lacustris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.4
-
 assay at Haematococcus lacustris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.20 Haematococcus lacustris isoelectric focusing, HpDGAT2D isozyme
-
 9.54
2.3.1.20 Haematococcus lacustris isoelectric focusing, HpDGAT2B isozyme
-
 9.6
2.3.1.20 Haematococcus lacustris isoelectric focusing, HpDGAT2E isozyme
-
 9.72
2.3.1.20 Haematococcus lacustris isoelectric focusing, HpDGAT2A isozyme
-
 9.86

General Information

EC Number General Information Comment Organism
2.3.1.20 metabolism diacylglycerol acyltransferase (DGAT) catalyzes the last step of the acyl-CoA-dependent triacylglycerol (TAG) biosynthesis and appears to represent a bottleneck in algal TAG formation. In Haematococcus pluvialis, the fatty acid composition of total lipids and TAG is changed when the algae cells are subjected to light stress, in which 16:0, 18:1, and 18:2 increase with the concomitant decreases in 18:3 Haematococcus lacustris