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Literature summary extracted from

  • Zufferey, R.
    In vitro assay to measure phosphatidylethanolamine methyltransferase activity (2016), J. Vis. Exp., 107, e53302 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.17 analysis simple cell-free enzymatic assay that measures the transfer of tritiated methyl group(s) from S-[methyl-3H]adenosyl-L-methionine onto phosphatidylethanolamine using whole cell extracts as an enzyme source. The resulting methylated forms of phosphatidylethanolamine are hydrophobic and can be separated from water soluble S-[methyl-3H]adenosyl-L-methionine by organic extraction Leishmania sp.

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.17 Leishmania sp.
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.17 S-adenosyl-L-methionine + phosphatidylethanolamine
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Leishmania sp. S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine
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