Literature summary extracted from
Li, G.; Wang, C.; Yang, M.; Cao, L.; Fu, D.; Liu, X.; Sun, D.; Chen, C.; Wang, Y.; Jia, Z.; Yang, C.; Guo, Y.; Rao, Z.
Structural insight into the African swine fever virus dUTPase reveals a novel folding pattern in the dUTPase family (2019), J. Virol., 94, e01698-19 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.6.1.23 |
expressed in Escherichia coli BL21(DE3) cells |
African swine fever virus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.6.1.23 |
in complex with dUMP and Mg2+, sitting drop vapor diffusion method, using 0.16 M sodium chloride, 0.08 M HEPES pH 7.5, 0.02 M sodium citrate tribasic dihydrate, 17% (w/v) polyethylene glycol 3350 |
African swine fever virus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.6.1.23 |
D33A |
the enzyme activity of the mutant is almost totally abolished |
African swine fever virus |
3.6.1.23 |
D91A |
the enzyme activity of the mutant is almost totally abolished |
African swine fever virus |
3.6.1.23 |
F154A |
the mutant exhibits approximately 10.5% of the activity of the wild type enzyme |
African swine fever virus |
3.6.1.23 |
Q120A |
the enzyme activity of the mutant is almost totally abolished |
African swine fever virus |
3.6.1.23 |
R71A |
the mutant exhibits approximately 9.6% of the activity of the wild type enzyme |
African swine fever virus |
3.6.1.23 |
S72A |
the mutant exhibits approximately 6.5% of the activity of the wild type enzyme |
African swine fever virus |
3.6.1.23 |
Y94A |
the mutant exhibits approximately 10% of the activity of the wild type enzyme |
African swine fever virus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.6.1.23 |
Mg2+ |
required |
African swine fever virus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.6.1.23 |
67000 |
- |
gel filtration |
African swine fever virus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
African swine fever virus |
- |
dUMP + diphosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.6.1.23 |
African swine fever virus |
A0A2X0SE53 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.6.1.23 |
Ni-NTA column chromatography and Superdex S200 gel filtration |
African swine fever virus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.6.1.23 |
dUTP + H2O |
- |
African swine fever virus |
dUMP + diphosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.6.1.23 |
homotrimer |
- |
African swine fever virus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.6.1.23 |
deoxyuridine 5'-triphosphate pyrophosphatase |
- |
African swine fever virus |
3.6.1.23 |
dUTPase |
- |
African swine fever virus |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.6.1.23 |
84 |
- |
melting temperature |
African swine fever virus |