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Literature summary extracted from

  • Li, G.; Wang, C.; Yang, M.; Cao, L.; Fu, D.; Liu, X.; Sun, D.; Chen, C.; Wang, Y.; Jia, Z.; Yang, C.; Guo, Y.; Rao, Z.
    Structural insight into the African swine fever virus dUTPase reveals a novel folding pattern in the dUTPase family (2019), J. Virol., 94, e01698-19 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli BL21(DE3) cells African swine fever virus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 in complex with dUMP and Mg2+, sitting drop vapor diffusion method, using 0.16 M sodium chloride, 0.08 M HEPES pH 7.5, 0.02 M sodium citrate tribasic dihydrate, 17% (w/v) polyethylene glycol 3350 African swine fever virus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 D33A the enzyme activity of the mutant is almost totally abolished African swine fever virus
3.6.1.23 D91A the enzyme activity of the mutant is almost totally abolished African swine fever virus
3.6.1.23 F154A the mutant exhibits approximately 10.5% of the activity of the wild type enzyme African swine fever virus
3.6.1.23 Q120A the enzyme activity of the mutant is almost totally abolished African swine fever virus
3.6.1.23 R71A the mutant exhibits approximately 9.6% of the activity of the wild type enzyme African swine fever virus
3.6.1.23 S72A the mutant exhibits approximately 6.5% of the activity of the wild type enzyme African swine fever virus
3.6.1.23 Y94A the mutant exhibits approximately 10% of the activity of the wild type enzyme African swine fever virus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required African swine fever virus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 67000
-
 gel filtration African swine fever virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O African swine fever virus
-
 dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 African swine fever virus A0A2X0SE53
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 Ni-NTA column chromatography and Superdex S200 gel filtration African swine fever virus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 African swine fever virus dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 homotrimer
-
 African swine fever virus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine 5'-triphosphate pyrophosphatase
-
 African swine fever virus
3.6.1.23 dUTPase
-
 African swine fever virus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.23 84
-
 melting temperature African swine fever virus