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Literature summary extracted from

  • Foong, C.; Lakshmanan, M.; Abe, H.; Taylor, T.; Foong, S.; Sudesh, K.
    A novel and wide substrate specific polyhydroxyalkanoate (PHA) synthase from unculturable bacteria found in mangrove soil (2018), J. Polym. Res., 25, 23 .
No PubMed abstract available

Application

EC Number Application Comment Organism
2.3.1.304 synthesis the PHA synthase is suitable for the biosynthesis of PHAs that can be used in various biomedical applications due to its ability to incorporate the lipase-degradable monomer sequences of 4-hydroxybutyrate (4HB) and 5-hydroxyvalerate (5HV) uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 BP-M-CPF4, DNA and amino acid sequence determination and analysis, functional assessment by in vivo PHA biosynthesis in a PHA-negative mutant, recombinant expression in the PHA-negative mutant of Cupriavidus necator under control of phaC1 promoter from Cupriavidus necator, subcloning in Escherichia coli uncultured bacterium
2.3.1.304 expressed in Cupriavidus necator strain PHB-4 uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 additional information recombinant phaC-deficient Cupriavidus necator expressing the enzyme is able to accumulate PHA homopolymers and copolymers including poly(3-hydroxybutyrate) [P(3HB)], poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], poly(3-hydroxybutyrate-co-5-hydroxyvalerate) [P(3HB-co-5HV)], poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3H4MV)], and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)], when suitable precursors are provided uncultured bacterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.304 cytoplasm
-
 uncultured bacterium 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n uncultured bacterium
-
 CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 additional information uncultured bacterium recombinant enzyme, expressed in phaC-deficient Cupriavidus necator, is able to accumulate PHA homopolymers and copolymers including poly(3-hydroxybutyrate) [P(3HB)], poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], poly(3-hydroxybutyrate-co-5-hydroxyvalerate) [P(3HB-co-5HV)], poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3H4MV)], and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)], when suitable precursors are provided ?
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-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 uncultured bacterium
-
 isolated from mangrove soil collected in the mangrove forest at Balik Pulau, Penang, Malaysia
-
2.3.1.304 uncultured bacterium
-
 uncultured bacterium from Balik Pulau (Penang, Malaysia) mangrove soil
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 (3R)-3-hydroxyacyl-CoA + poly[(R)-3-hydroxyalkanoate]n
-
 uncultured bacterium CoA + poly[(R)-3-hydroxyalkanoate]n+1
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxy-4-methylvalerate]
-
 uncultured bacterium CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate]
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyhexanoate]
-
 uncultured bacterium CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyhexanoate]
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[(R)-3-hydroxyvalerate]
-
 uncultured bacterium CoA + poly[(R)-3-hydroxybutyrate-co-3-hydroxyvalerate]
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[4-hydroxybutyrate]
-
 uncultured bacterium CoA + poly[(R)-3-hydroxybutyrate-co-4-hydroxybutyrate]
-
 ?
2.3.1.304 (3R)-3-hydroxybutyryl-CoA + poly[5-hydroxyvalerate]
-
 uncultured bacterium CoA + poly[(R)-3-hydroxybutyrate-co-5-hydroxyvalerate]
-
 ?
2.3.1.304 additional information recombinant enzyme, expressed in phaC-deficient Cupriavidus necator, is able to accumulate PHA homopolymers and copolymers including poly(3-hydroxybutyrate) [P(3HB)], poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)], poly(3-hydroxybutyrate-co-5-hydroxyvalerate) [P(3HB-co-5HV)], poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)], poly(3-hydroxybutyrate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3H4MV)], and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(3HB-co-3HHx)], when suitable precursors are provided uncultured bacterium ?
-
-
2.3.1.304 additional information the polyhydroxyalkanoate (PHA) synthase (PhaC) from a mangrove soil metagenome possesses a very wide substrate specificity. The enzyme shows the ability to incorporate six types of PHA monomers, 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), 3-hydroxy-4-methylvalerate (3H4MV), 5-hydroxyvalerate (5HV) and 3-hydroxyhexanoate (3HHx) in the presence of suitable precursors uncultured bacterium ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 BP-M-CPF4
-
 uncultured bacterium
2.3.1.304 PHA synthase
-
 uncultured bacterium
2.3.1.304 PhaC
-
 uncultured bacterium
2.3.1.304 polyhydroxyalkanoate synthase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.304 30
-
 in vivo assay at uncultured bacterium

General Information

EC Number General Information Comment Organism
2.3.1.304 physiological function PhaC can polymerize high molecular weight hydrophobic PHA chains in the hydrophilic environment of the cell cytoplasm uncultured bacterium