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Literature summary extracted from

  • Choi, G.H.; Lee, H.Y.; Back, K.
    Chloroplast overexpression of rice caffeic acid O-methyltransferase increases melatonin production in chloroplasts via the 5-methoxytryptamine pathway in transgenic rice plants (2017), J. Pineal Res., 63, e12412 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expressed in Escherichia coli BL21(DE3) cells and calli from Oryza sativa cultivar Dongjin Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.68 chloroplast
-
 Oryza sativa 9507
-
2.1.1.68 cytoplasm
-
 Oryza sativa 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + caffeic acid Oryza sativa
-
 S-adenosyl-L-homocysteine + ferulic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + N-acetylserotonin Oryza sativa
-
 S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.68 S-adenosyl-L-methionine + quercetin Oryza sativa
-
 S-adenosyl-L-homocysteine + isorhamnetin
-
 ?
2.1.1.68 S-adenosyl-L-methionine + serotonin Oryza sativa
-
 S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.68 Ni-NTA column chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.68 seedling
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + caffeic acid
-
 Oryza sativa S-adenosyl-L-homocysteine + ferulic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + N-acetylserotonin
-
 Oryza sativa S-adenosyl-L-homocysteine + melatonin
-
 ?
2.1.1.68 S-adenosyl-L-methionine + quercetin
-
 Oryza sativa S-adenosyl-L-homocysteine + isorhamnetin
-
 ?
2.1.1.68 S-adenosyl-L-methionine + serotonin
-
 Oryza sativa S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.68 ? x * 38000, N-terminal His-tagged native protein, SDS-PAGE Oryza sativa
2.1.1.68 ? x * 44000, recombinant protein, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 caffeic acid O-methyltransferase
-
 Oryza sativa
2.1.1.68 COMT
-
 Oryza sativa