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Literature summary extracted from

  • Lee, H.L.; Kim, S.Y.; Kim, E.J.; Han, D.Y.; Kim, B.G.; Ahn, J.H.
    Synthesis of methylated anthranilate derivatives using engineered strains of Escherichia coli (2019), J. Microbiol. Biotechnol., 29, 839-844.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.111 synthesis expression of anthranilate N-methyltransferase, anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and anthranilate coenzyme A ligase from Pseudomonas aeruginosa in Escherichia coli mutants mutants (metJ, trpD, tyrR), which provide more anthranilate and/or S-adenosyl methionine, to synthesize N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate. Approximately, 185.3 microM N-methylanthranilate and 95.2 microM methyl N-methylanthranilate are synthesized Ruta graveolens
2.1.1.111 synthesis three anthranilate derivatives, N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate are synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and pqsA encoding anthranilate coenzyme A ligase from Pseudomonas aeruginosa are cloned and Escherichia coli strains harboring these genes were used to synthesize the three desired compounds. Escherichia coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, are used to increase the production of the synthesized compounds. 0.1853 mM N-methylanthranilate and 0.0952 mM methyl N-methylanthranilate are synthesized Ruta graveolens
2.3.1.232 synthesis three anthranilate derivatives, N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate are synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and pqsA encoding anthranilate coenzyme A ligase from Pseudomonas aeruginosa are cloned and Eschetrichia coli strains harboring these genes were used to synthesize the three desired compounds. Escherichia coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, are used to increase the production of the synthesized compounds. 0.1853 mM N-methylanthranilate and 0.0952 mM methyl N-methylanthranilate are synthesized Vitis labrusca
6.2.1.32 synthesis three anthranilate derivatives, N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate are synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and pqsA encoding anthranilate coenzyme A ligase from Pseudomonas aeruginosa are cloned and Eschetrichia coli strains harboring these genes were used to synthesize the three desired compounds. Escherichia coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, are used to increase the production of the synthesized compounds. 0.1853 mM N-methylanthranilate and 0.0952 mM methyl N-methylanthranilate are synthesized Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.111 expression in Escherichia coli Ruta graveolens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.111 Ruta graveolens A9X7L0
-
-
2.3.1.232 Vitis labrusca Q3ZPN4
-
-
6.2.1.32 Pseudomonas aeruginosa Q9I4X3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.111 S-adenosyl-L-methionine + anthranilate
-
 Ruta graveolens S-adenosyl-L-homocysteine + N-methylanthranilate
-
 ?
2.1.1.111 S-adenosyl-L-methionine + methyl anthranilate
-
 Ruta graveolens S-adenosyl-L-homocysteine + methyl N-methylanthranilate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.111 NMT
-
 Ruta graveolens
2.3.1.232 AMAT
-
 Vitis labrusca
2.3.1.232 anthraniloyl-coenzyme A:methanol acyltransferase
-
 Vitis labrusca
6.2.1.32 PqsA
-
 Pseudomonas aeruginosa