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Literature summary extracted from

  • Kim, E.J.; Cha, M.N.; Kim, B.G.; Ahn, J.H.
    Production of curcuminoids in engineered Escherichia coli (2017), J. Microbiol. Biotechnol., 27, 975-982 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.211 synthesis production of curcuminoids in engineered Escherichia coli. PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS (curcumin synthase) genes, are introduced into Escherichia coli, and each strain produces dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in Escherichia coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, is engineered. Using the engineered strains, the production of bisdemethoxycurcumin increases from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l Oryza sativa Japonica Group
2.3.1.211 synthesis production of curcuminoids in engineered Escherichia coli. Two curcuminoids (dicinnamoylmethane and bisdemethoxycurcumin) are synthesized from glucose in Escherichia coli. PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS genes, are introduced into Escherichia coli, and each strain produces dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in Escherichia coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, is engineered. Using the engineered strains, the production of bisdemethoxycurcumin increases from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l Oryza sativa Japonica Group
2.3.1.217 synthesis production of curcuminoids in engineered Escherichia coli. Two curcuminoids (dicinnamoylmethane and bisdemethoxycurcumin) are synthesized from glucose in Escherichia coli. PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS (curcumin synthase) genes, are introduced into Escherichia coli, and each strain produces dicinnamoylmethane or bisdemethoxycurcumin, respectively. In order to increase the production of curcuminoids in Escherichia coli, the shikimic acid biosynthesis pathway, which increases the substrates for curcuminoid biosynthesis, is engineered. Using the engineered strains, the production of bisdemethoxycurcumin increases from 0.32 to 4.63 mg/l, and that of dicinnamoylmethane from 1.24 to 6.95 mg/l Oryza sativa Japonica Group

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.211 PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS genes, are introduced into Escherichia coli Oryza sativa Japonica Group
2.3.1.217 PAL (phenylalanine ammonia lyase) or TAL (tyrosine ammonia lyase), along with Os4CL (p-coumaroyl-CoA ligase) and CUS (curcumin synthase) genes, are introduced into Escherichia coli Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.211 Oryza sativa Japonica Group Q8LIL0
-
-
2.3.1.217 Oryza sativa Japonica Group Q8LIL0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.211 2 4-coumaroyl-CoA + malonyl-CoA + H2O
-
 Oryza sativa Japonica Group 3 CoA + bisdemethoxycurcumin + 2 CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.211 curcumin synthase
-
 Oryza sativa Japonica Group
2.3.1.211 CUS
-
 Oryza sativa Japonica Group
2.3.1.211 OsCUS
-
 Oryza sativa Japonica Group
2.3.1.217 OsCUS
-
 Oryza sativa Japonica Group