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Literature summary extracted from

  • Zhu, L.; Jiang, D.; Zhou, Y.; Lu, Y.; Fan, Y.; Chen, X.
    Batch-feeding whole-cell catalytic synthesis of alpha-arbutin by amylosucrase from Xanthomonas campestris (2019), J. Ind. Microbiol. Biotechnol., 46, 759-767 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.4 biotechnology arbutin as a safe hydroquinone derivative is one of most important skin-whitening ingredients including beta-arbutin and alpha-arbutin. The batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate Xanthomonas campestris pv. campestris
2.4.1.4 synthesis the batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate Xanthomonas campestris pv. campestris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.4 expression in Escherichia coli Xanthomonas campestris pv. campestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.4 Ba2+ 1.0 mM, 12% inhibition Xanthomonas campestris pv. campestris
2.4.1.4 Cu2+ 1.0 mM, complete inhibition Xanthomonas campestris pv. campestris
2.4.1.4 Mn2+ 1.0 mM, 50% inhibition Xanthomonas campestris pv. campestris
2.4.1.4 Ni2+ 1.0 mM, 30% inhibition Xanthomonas campestris pv. campestris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.4 9.58
-
 hydroquinone pH 7.0, 30°C Xanthomonas campestris pv. campestris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.4 Fe2+ 1.0 mM, 40% activation Xanthomonas campestris pv. campestris
2.4.1.4 K+ 1.0 mM, 10% activation Xanthomonas campestris pv. campestris

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Xanthomonas campestris pv. campestris A0A0H2X5U3
-
-
2.4.1.4 Xanthomonas campestris pv. campestris 8004 A0A0H2X5U3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.4
-
 Xanthomonas campestris pv. campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 sucrose + hydroquinone
-
 Xanthomonas campestris pv. campestris D-fructose + alpha-arbutin
-
 ?
2.4.1.4 sucrose + hydroquinone
-
 Xanthomonas campestris pv. campestris 8004 D-fructose + alpha-arbutin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.4 Amy-1
-
 Xanthomonas campestris pv. campestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.4 35
-
-
 Xanthomonas campestris pv. campestris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.4 20 40 20°C: about 60% of maximal activity, 40°C: about 70% of maximal activity Xanthomonas campestris pv. campestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.4 30
-
 high stability, maintaining 83% activity after 2 h Xanthomonas campestris pv. campestris
2.4.1.4 50
-
 complete loss of catalytic activity after 30 min Xanthomonas campestris pv. campestris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.4 23.9
-
 hydroquinone pH 7.0, 30°C Xanthomonas campestris pv. campestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 7.5
-
-
 Xanthomonas campestris pv. campestris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.4 5 8.5 pH 5.0: 65% of maximal activity, pH 8.0: 45% of maximal activity Xanthomonas campestris pv. campestris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.4 2.5
-
 hydroquinone pH 7.0, 30°C Xanthomonas campestris pv. campestris