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Literature summary extracted from
- Purification and characterization of a flavor-related enzyme, gamma-glutamyl-transpeptidase, from Toona sinensis leaves (2016), J. Hortic. Sci. Biotechnol., 91, 611-618 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.2.2 | Fe3+ | strong activation | Toona sinensis |  |
| 2.3.2.2 | K+ | slight activation | Toona sinensis | |
| 2.3.2.2 | Mn2+ | slight activation | Toona sinensis | |
| 3.4.19.13 | Fe3+ | strong activation | Toona sinensis | |
| 3.4.19.13 | K+ | slight activation | Toona sinensis | |
| 3.4.19.13 | Mn2+ | slight activation | Toona sinensis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.2.2 | Ca2+ | slight inhibition | Toona sinensis | |
| 2.3.2.2 | Cu2+ | strong inhibition | Toona sinensis | |
| 2.3.2.2 | Mg2+ | slight inhibition | Toona sinensis | |
| 2.3.2.2 | Na+ | slight inhibition | Toona sinensis | |
| 2.3.2.2 | Zn2+ | strong inhibition | Toona sinensis | |
| 3.4.19.13 | Ca2+ | slight inhibition | Toona sinensis | |
| 3.4.19.13 | Cu2+ | strong inhibition | Toona sinensis | |
| 3.4.19.13 | Mg2+ | slight inhibition | Toona sinensis | |
| 3.4.19.13 | Na+ | slight inhibition | Toona sinensis | |
| 3.4.19.13 | Zn2+ | strong inhibition | Toona sinensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.2.2 | 3.6 | - |
L-gamma-glutamyl-4-nitroanilide | pH 8.0, 37°C | Toona sinensis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.2 | 90100 | - |
gel filtration | Toona sinensis |
| 3.4.19.13 | 90100 | - |
gel filtration | Toona sinensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.13 | (S,S)-gamma-glutamyl-(cis-S-1-propenyl)-thioglycine + H2O | Toona sinensis | - |
(S)-(cis-S-1-propenyl)thioglycine + L-glutamate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.2.2 | Toona sinensis | - |
- |
- |
| 3.4.19.13 | Toona sinensis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.2.2 | native enzyme 40.7fold from leaves by ammonium sulfate fractionation, dialysis, and gel filtration | Toona sinensis |
| 3.4.19.13 | native enzyme 40.7fold from leaves by ammonium sulfate fractionation, dialysis, and gel filtration | Toona sinensis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.3.2.2 | leaf | - |
Toona sinensis | - |
| 3.4.19.13 | leaf | - |
Toona sinensis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.2 | 8.92 | - |
purified enzyme, pH 8.0, 37°C | Toona sinensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.2.2 | (S,S)-gamma-glutamyl-(cis-S-1-propenyl)-thioglycine + ? | - |
Toona sinensis | (S)-(cis-S-1-propenyl)thioglycine + L-glutamate | - |
? | |
| 2.3.2.2 | L-gamma-glutamyl-4-nitroanilide + glycylglycine | - |
Toona sinensis | 4-nitroaniline + 5-L-glutamyl-glycylglycine | - |
? | |
| 3.4.19.13 | (S,S)-gamma-glutamyl-(cis-S-1-propenyl)-thioglycine + H2O | - |
Toona sinensis | (S)-(cis-S-1-propenyl)thioglycine + L-glutamate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.3.2.2 | heterodimer | 1 * 64000 + 1 * 26000, SDS-PAGE | Toona sinensis |
| 3.4.19.13 | heterodimer | 1 * 64000 + 1 * 26000, SDS-PAGE | Toona sinensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.3.2.2 | gamma-glutamyl-transpeptidase | - |
Toona sinensis |
| 2.3.2.2 | GGT | - |
Toona sinensis |
| 2.3.2.2 | More | see also EC 3.4.19.13 | Toona sinensis |
| 3.4.19.13 | gamma-glutamyl-transpeptidase | - |
Toona sinensis |
| 3.4.19.13 | GGT | - |
Toona sinensis |
| 3.4.19.13 | More | see also EC 2.3.2.2 | Toona sinensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.2 | 60 | - |
- |
Toona sinensis |
| 3.4.19.13 | 60 | - |
- |
Toona sinensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.2 | 20 | 70 | activity range, optimum activity at 70°C, relative activities at 30°C and 70°C are approximately 88.86% and 92.75% of the maximal activity, respectively | Toona sinensis |
| 3.4.19.13 | 20 | 70 | activity range, optimum activity at 70°C, relative activities at 30°C and 70°C are approximately 88.86% and 92.75% of the maximal activity, respectively | Toona sinensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.2 | 20 | 70 | purified native enzyme, the enzyme retains more than 88% of its activity at 20-70°C, but the enzyme activity is significantly decreased at temperatures above 70°C | Toona sinensis |
| 3.4.19.13 | 20 | 70 | purified native enzyme, the enzyme retains more than 88% of its activity at 20-70°C, but the enzyme activity is significantly decreased at temperatures above 70°C | Toona sinensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.2 | 7 | - |
- |
Toona sinensis |
| 3.4.19.13 | 7 | - |
- |
Toona sinensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.2 | 3 | 10 | optimum activity at pH 7.0, 80% of maximal activity at pH 6.5-8.0, and residual activity sharply decreased to less than 50% at above pH 10.0 or below pH 4.0 | Toona sinensis |
| 3.4.19.13 | 3 | 10 | optimum activity at pH 7.0, 80% of maximal activity at pH 6.5-8.0, and residual activity sharply decreased to less than 50% at above pH 10.0 or below pH 4.0 | Toona sinensis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.3.2.2 | physiological function | gamma-glutamyl transpeptidase (GGT) is a widely distributed enzyme from bacteria to plants and mammals that catalyzes the cleavage of the gamma-glutamyl linkage of gamma-glutamyl compounds, such as glutathione and transfer of the gamma-glutamyl residue either to amino acid or peptides (transpeptidation) or water (hydrolysis, EC 3.4.19.13). GGT catalyzes the release of the glutamic acid moiety from (S,S)-gamma-glutamyl-(cis-S-1-propenyl)-thioglycine, a flavor precursor found in Toona sinensis. Toona sinensis shoots and young leaves with unique aroma are consumed as a delicious seasonal vegetable in China. GGT may play an important role in the formation of volatile sulfur-containing compounds, including propene thiol which determines the characteristic aroma of Toona sinensis vegetables | Toona sinensis |
| 3.4.19.13 | physiological function | gamma-glutamyl transpeptidase (GGT) is a widely distributed enzyme from bacteria to plants and mammals that catalyzes the cleavage of the gamma-glutamyl linkage of gamma-glutamyl compounds, such as glutathione and transfer of the gamma-glutamyl residue either to amino acid or peptides (transpeptidation, EC 2.3.2.2) or water (hydrolysis). GGT catalyzes the release of the glutamic acid moiety from (S,S)-gamma-glutamyl-(cis-S-1-propenyl)-thioglycine, a flavor precursor found in Toona sinensis. Toona sinensis shoots and young leaves with unique aroma are consumed as a delicious seasonal vegetable in China. GGT may play an important role in the formation of volatile sulfur-containing compounds, including propene thiol which determines the characteristic aroma of Toona sinensis vegetables | Toona sinensis |
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