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Literature summary extracted from

  • Patel, N.; Pirani, K.A.; Zhu, T.; Cheung-See-Kit, M.; Lee, S.; Chen, D.G.; Zufferey, R.
    The glycerol-3-phosphate acyltransferase TbGAT is dispensable for viability and the synthesis of glycerolipids in Trypanosoma brucei (2016), J. Eukaryot. Microbiol., 63, 598-609 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 gene Tb10.70.4400, sequence comaprisons, TbGAT restores glycerol-3-phosphate acyltransferase activity when expressed in a Leishmania major deletion strain lacking this activity and exhibits preference for medium length, unsaturated fatty acyl-CoAs Trypanosoma brucei brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.15 additional information creation of the null mutant DELTAtbgat/DELTA tbgat and complementation of lines DELTA tbgat/DELTA tbgat/TbGAT and DELTA tbgat/DELTA tbgat/HV:TbGAT. The null mutant of TbGAT is created by sequential replacement of both alleles with first a BSD and then a HYG cassette, conferring blasticidin and hygromycin resistance, respectively, geno- and phenotypes, overview Trypanosoma brucei brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 endoplasmic reticulum membrane TbGAT localizes to the endoplasmic reticulum membrane with its N-terminal domain facing the cytosol Trypanosoma brucei brucei 5789
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Trypanosoma brucei brucei
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Trypanosoma brucei brucei 927/4 GUTat10.1
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Trypanosoma brucei brucei Q38BT6
-
-
2.3.1.15 Trypanosoma brucei brucei 927/4 GUTat10.1 Q38BT6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 additional information procyclic form Trypanosoma brucei brucei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Trypanosoma brucei brucei CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate the enzyme exhibits preference for medium length, unsaturated fatty acyl-CoAs, i.e. palmitoyl-CoA Trypanosoma brucei brucei CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Trypanosoma brucei brucei 927/4 GUTat10.1 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate the enzyme exhibits preference for medium length, unsaturated fatty acyl-CoAs, i.e. palmitoyl-CoA Trypanosoma brucei brucei 927/4 GUTat10.1 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Trypanosoma brucei brucei CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Trypanosoma brucei brucei 927/4 GUTat10.1 CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 More TbGAT (gene ID Tb927.10.3100) encodes a protein of 698 amino acids with three putative C-terminal hydrophobic spans. No signal peptide can be predicted Trypanosoma brucei brucei

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase
-
 Trypanosoma brucei brucei
2.3.1.15 TbGAT
-
 Trypanosoma brucei brucei

General Information

EC Number General Information Comment Organism
2.3.1.15 malfunction the enzyme null mutant exhibits a slight decrease in phosphatidylethanolamine biosynthesis that is compensated with a modest increase in production of ether phosphatidylcholine. TbGAT null mutant in Trypanosoma brucei procyclic forms lacks glycerol-3-phosphate acyltransferase activity, but remains viable and exhibits similar growth rate as the wild-type Trypanosoma brucei brucei
2.3.1.15 physiological function the biosynthesis of ester glycerolipids initiates with the stepwise addition of fatty acyl groups to glycerol-3-phosphate (G3P). The first fatty acyl group is added at the sn-1 position by a G3P acyltransferase (GPAT) to give 1-acyl-G3P or lysophosphatidic acid (LPA), while the second fatty acyl group is added to the sn-2 position by a 1-acyl-G3P acyltransferase to give phosphatidic acid (PA). The glycerol-3-phosphate acyltransferase TbGAT is dispensable for viability and the biosynthesis of glycerolipids, i.e. phosphatidylcholine, phosphatidylinositol, phosphatidylserine, and GPI-anchored protein procyclin, in Trypanosoma brucei. Recombinant TbGAT restores glycerol-3-phosphate acyltransferase activity when expressed in a Leishmania major deletion strain lacking this activity and exhibits preference for medium length, unsaturated fatty acyl-CoAs. Gene TbGAT encodes a GPAT enzyme with broad fatty acyl-CoA specificity but preference for oleoyl-CoA Trypanosoma brucei brucei