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Literature summary extracted from

  • Zhang, J.; Zhang, Y.; Li, M.
    High-level secretion and characterization of cyclodextrin glycosyltransferase in recombinant Komagataella phaffii (2017), J. Biotechnol., 259, 126-134 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Komagataella phaffii Alkalihalobacillus pseudalcaliphilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Alkalihalobacillus pseudalcaliphilus
-
-
-
2.4.1.19 Alkalihalobacillus pseudalcaliphilus 8SB
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 ammonium sulfate precipitation Alkalihalobacillus pseudalcaliphilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 starch + glycosyl acceptor
-
 Alkalihalobacillus pseudalcaliphilus beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 starch + glycosyl acceptor
-
 Alkalihalobacillus pseudalcaliphilus 8SB beta-cyclodextrin + gamma-cyclodextrin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 ? x * 71000, SDS-PAGE Alkalihalobacillus pseudalcaliphilus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 beta-CGTase
-
 Alkalihalobacillus pseudalcaliphilus
2.4.1.19 cyclodextrin glucosyltransferase
-
 Alkalihalobacillus pseudalcaliphilus
2.4.1.19 gamma-CGTase
-
 Alkalihalobacillus pseudalcaliphilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 50
-
-
 Alkalihalobacillus pseudalcaliphilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.19 40 70 the beta-cyclodextrin forming activity decreases to 94.6% and 89.2% after 1 and 2 h at 50°C, respectively, and decreases to 17.6% after 1 h at 70°C. After 2 h at 60°C and 60°C-70°C, the beta-cyclodextrin forming activity sharply decreases to 6% and 2.3%, respectively. After 1 and 2 h at 40°C or 50°C, the gamma-cyclodextrin forming activity slightly decreases. With the increase in temperature to 70°C, the gamma-cyclodextrin forming activity decreases to 15.2% after 1 h of treatment. Moreover, the gamma-cyclodextrin forming activity decreases to 8.9% and 2.1% after 2 h of treatment at 60°C and 70°C, respectively Alkalihalobacillus pseudalcaliphilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 7 8 pH optimum for gamma-cyclodextrin production Alkalihalobacillus pseudalcaliphilus
2.4.1.19 9
-
 pH optimum for beta-cyclodextrin production Alkalihalobacillus pseudalcaliphilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.19 6 10 the beta-cyclodextrin forming activity decreases with decreasing pH from 6.0 to 4.0, and increases with increasing pH from 10.0 to 11.0. However, there is no significant difference in the beta-cyclodextrin forming activity after 1 and 2 h of treatment at pH 6.0-10.0. With regard to gamma-cyclodextrin production, the enzyme activity increases with increasing pH from 4.0 to 8.0, and then decreases to 77.2% and 66.4% after 1 and 2 h at pH 11.0, respectively Alkalihalobacillus pseudalcaliphilus