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Literature summary extracted from

  • Li, D.W.; Cen, S.Y.; Liu, Y.H.; Balamurugan, S.; Zheng, X.Y.; Alimujiang, A.; Yang, W.D.; Liu, J.S.; Li, H.Y.
    A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica (2016), J. Biotechnol., 229, 65-71 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene DGAT2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and phylogenetic analysis, recombinant overexpression of FLAG-tagged enzyme DGAT2 in Nannochloropsis oceanica cells, real-time quantitative PCR expression analysis Nannochloropsis oceanica

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information generation of DGAT2 overexpressing cells of Nannochloropsis oceanica, expression analysis and lipid content determination, overview. The introduced transgene DGAT2 is successfully integrated,transcribed and translated in the transformed algal cells. The overexpressed DGAT2 does not have apparent effects on the growth and biomass accumulation of the engineered microalgae, analysis of neutral lipid content. There is a variation in terms of fatty acid composition between the engineered and wild-type cells. Saturated fatty acid (SFAs) content is significantly increased by 53.1% in engineered lines compared to wild-type. On the other hand,monounsaturated fatty acids (MUFAs) decrease by 52.9% in engineered cells. Similarly, polyunsaturated fatty acids (PUFAs) show an apparent decrease of 74.6%, including arachidonic acid (C20:4) and EPA (C20:5) in engineered cells Nannochloropsis oceanica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Nannochloropsis oceanica
-
CoA + 1,2,3-triacylglycerol
-
?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Nannochloropsis oceanica CCAP 849/10
-
CoA + 1,2,3-triacylglycerol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Nannochloropsis oceanica A0A191M6B9 formerly CCMP1779
-
2.3.1.20 Nannochloropsis oceanica CCAP 849/10 A0A191M6B9 formerly CCMP1779
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
Nannochloropsis oceanica CoA + 1,2,3-triacylglycerol
-
?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
Nannochloropsis oceanica CCAP 849/10 CoA + 1,2,3-triacylglycerol
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 ? x * 43000, recombinant FLAG-tagged enzyme, SDS-PAGE Nannochloropsis oceanica

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 2 diacylglycerol acyltransferase
-
Nannochloropsis oceanica
2.3.1.20 DGAT2
-
Nannochloropsis oceanica

General Information

EC Number General Information Comment Organism
2.3.1.20 physiological function the type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica Nannochloropsis oceanica