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Literature summary extracted from

  • Kuhaudomlarp, S.; Patron, N.; Henrissat, B.; Rejzek, M.; Saalbach, G.; Field, R.
    Identification of Euglena gracilis beta-1,3-glucan phosphorylase and establishment of a new glycoside hydrolase (GH) family GH149 (2018), J. Biol. Chem., 293, 2865-2876 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.97 expression in Escherichia coli Euglena gracilis var. saccharophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.97 1-deoxynojirimycin weak competitive inhibition Euglena gracilis var. saccharophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.97 0.56
-
 alpha-D-glucose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 0.67
-
 laminaribiose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.26
-
 laminaritriose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.41
-
 laminaritetraose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 2.29
-
 Laminaripentaose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 2.88
-
 Laminarihexaose pH 7.0, 30°C Euglena gracilis var. saccharophila

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.97 Euglena gracilis var. saccharophila A0A2I6SUQ9 glycoside hydrolase family GH149
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.97 alpha-D-glucose + glucose 1-phosphate
-
 Euglena gracilis var. saccharophila phosphate + laminaribiose
-
 r
2.4.1.97 laminaribiose + glucose 1-phosphate
-
 Euglena gracilis var. saccharophila phosphate + laminaritriose
-
 r
2.4.1.97 laminarihexaose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis var. saccharophila laminariheptaose + phosphate
-
 r
2.4.1.97 laminaripentaose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis var. saccharophila laminarihexaose + phosphate
-
 r
2.4.1.97 laminaritetraose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis var. saccharophila laminaripentaose + phosphate
-
 r
2.4.1.97 laminaritriose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis var. saccharophila laminaritetraose + phosphate
-
 r
2.4.1.97 laminaritriose + phosphate
-
 Euglena gracilis var. saccharophila laminaribiose + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.97 additional information protein is able to catalyze the conversion of glucose and glucose 1-phosphate into oligosaccharides with degree of polymerization up to 12 glucose units. Tps3 acts exclusively on glucose-beta-1,3-glucose oligosaccharides.The activity is dependent on both glucose and glucose 1-phosphate as substrates, excluding either from the reaction mixture completely abolishes oligosaccharide production Euglena gracilis var. saccharophila ?
-
-

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.97 30 40
-
 Euglena gracilis var. saccharophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.97 1.08
-
 laminaribiose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.1
-
 alpha-D-glucose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.1
-
 Laminarihexaose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.12
-
 laminaritriose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.12
-
 laminaritetraose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.13
-
 Laminaripentaose pH 7.0, 30°C Euglena gracilis var. saccharophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.97 5 8
-
 Euglena gracilis var. saccharophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.97 0.38
-
 Laminarihexaose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 0.5
-
 Laminaripentaose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 0.79
-
 laminaritetraose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 0.89
-
 laminaritriose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.62
-
 laminaribiose pH 7.0, 30°C Euglena gracilis var. saccharophila
2.4.1.97 1.99
-
 alpha-D-glucose pH 7.0, 30°C Euglena gracilis var. saccharophila