Literature summary extracted from

Shaw, J.A.; Henard, C.A.; Liu, L.; Dieckman, L.M.; Vazquez-Torres, A.; Bourret, T.J.
Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway (2018), J. Biol. Chem., 293, 11271-11282 .

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.2.1.1	0.023	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.058	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.082	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.099	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.12	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.17	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.18	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.27	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.29	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.66	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.73	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	0.75	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.2.1.1	D-erythrose 4-phosphate + D-xylulose 5-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium	-	?	-	r
2.2.1.1	D-erythrose 4-phosphate + D-xylulose 5-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	-	?	-	r
2.2.1.1	D-ribose 5-phosphate + D-xylulose 5-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
2.2.1.1	D-ribose 5-phosphate + D-xylulose 5-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	-	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
2.2.1.1	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium	-	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r
2.2.1.1	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	-	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.2.1.1	Salmonella enterica subsp. enterica serovar Typhimurium	A0A0F6B484 and A0A0F6B483	-	-
2.2.1.1	Salmonella enterica subsp. enterica serovar Typhimurium	A0A0F6B4M5	-	-
2.2.1.1	Salmonella enterica subsp. enterica serovar Typhimurium	A0A0F6B6H7	-	-
2.2.1.1	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	A0A0F6B484 and A0A0F6B483	-	-
2.2.1.1	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	A0A0F6B4M5	-	-
2.2.1.1	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	A0A0F6B6H7	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.2.1.1	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	r
2.2.1.1	D-erythrose 4-phosphate + D-xylulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	?	-	r
2.2.1.1	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
2.2.1.1	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	r
2.2.1.1	additional information	isoform TktA is responsible for more than 88% of the transketolase activity in wild type cells	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	-
2.2.1.1	additional information	isoform TktA is responsible for more than 88% of the transketolase activity in wild type cells	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	?	-	-
2.2.1.1	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r
2.2.1.1	sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028s	D-ribose 5-phosphate + D-xylulose 5-phosphate	-	r

Subunits

EC Number	Subunits	Comment	Organism
2.2.1.1	?	x * 34000, isoform TktC, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	?	x * 72000, isoform TktA, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	?	x * 73000, isoform TktB, SDS-PAGE	Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number	Synonyms	Comment	Organism
2.2.1.1	STM14_2885	-	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	STM14_2886	-	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	TktA	isoform	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	TktB	isoform	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	TKTc	isoform	Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.2.1.1	671	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	816	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	1350	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	1390	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	52500	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	67100	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	71400	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	78500	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	86500	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	95400	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	104000	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	131000	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.2.1.1	thiamine diphosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium

General Information

EC Number	General Information	Comment	Organism
2.2.1.1	malfunction	transketolase-deficient Salmonella enterica Typhimurium can grow with glucose, but not ribose, as the sole carbon source	Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.2.1.1	5580	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	10800	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	23200	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	60700	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	102000	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	106000	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	131000	-	D-ribose 5-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	269000	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	299000	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	422000	-	D-xylulose 5-phosphate	with excess D-erythrose 4-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	477000	-	D-xylulose 5-phosphate	with excess D-ribose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium
2.2.1.1	1050000	-	D-erythrose 4-phosphate	with excess D-xylose 5-phosphate as cosubstrate, at pH 7.5 and 37°C	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)