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Literature summary extracted from

  • Diez, M.; Miah, F.; Stevenson, C.; Lawson, D.; Iglesias, A.; Bornemann, S.
    The production and utilization of GDP-glucose in the biosynthesis of trehalose 6-phosphate by Streptomyces venezuelae (2017), J. Biol. Chem., 292, 945-954 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.36 expressed in Escherichia coli SoluBL21 cells Streptomyces venezuelae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.36 0.12
-
 GDP-glucose pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 0.44
-
 ADP-glucose pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 0.9
-
 glucose 6-phosphate with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 0.93
-
 glucose 6-phosphate with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication Streptomyces venezuelae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.36 109000
-
 gel filtration Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.36 GDP-glucose + glucose 6-phosphate Streptomyces venezuelae
-
 GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.36 GDP-glucose + glucose 6-phosphate Streptomyces venezuelae ATCC 10712
-
 GDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.36 Streptomyces venezuelae F2RH01
-
-
2.4.1.36 Streptomyces venezuelae ATCC 10712 F2RH01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.36 nickel affinity column chromatography and Superdex 200 gel filtration Streptomyces venezuelae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.36 ADP-glucose + glucose 6-phosphate
-
 Streptomyces venezuelae ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.36 ADP-glucose + glucose 6-phosphate
-
 Streptomyces venezuelae ATCC 10712 ADP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.36 GDP-glucose + glucose 6-phosphate
-
 Streptomyces venezuelae GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.36 GDP-glucose + glucose 6-phosphate the enzyme has a preference for GDP-glucose Streptomyces venezuelae GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.36 GDP-glucose + glucose 6-phosphate
-
 Streptomyces venezuelae ATCC 10712 GDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.36 GDP-glucose + glucose 6-phosphate the enzyme has a preference for GDP-glucose Streptomyces venezuelae ATCC 10712 GDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.36 homodimer 2 * 51000, calculated from amino acid sequence Streptomyces venezuelae

Synonyms

EC Number Synonyms Comment Organism
2.4.1.36 alpha,alpha-trehalose-phosphate synthase
-
 Streptomyces venezuelae
2.4.1.36 OtsA
-
 Streptomyces venezuelae
2.4.1.36 trehalose-6-phosphate synthase
-
 Streptomyces venezuelae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.36 26
-
 glucose 6-phosphate with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 29
-
 ADP-glucose pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 123
-
 glucose 6-phosphate with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 139
-
 GDP-glucose pH and temperature not specified in the publication Streptomyces venezuelae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.36 29
-
 glucose 6-phosphate with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 67
-
 ADP-glucose pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 132
-
 glucose 6-phosphate with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication Streptomyces venezuelae
2.4.1.36 1143
-
 GDP-glucose pH and temperature not specified in the publication Streptomyces venezuelae