EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.36 | expressed in Escherichia coli SoluBL21 cells | Streptomyces venezuelae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.36 | 0.12 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 0.44 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 0.9 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 0.93 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.36 | 109000 | - |
gel filtration | Streptomyces venezuelae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.36 | GDP-glucose + glucose 6-phosphate | Streptomyces venezuelae | - |
GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.36 | GDP-glucose + glucose 6-phosphate | Streptomyces venezuelae ATCC 10712 | - |
GDP + alpha,alpha-trehalose 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.36 | Streptomyces venezuelae | F2RH01 | - |
- |
2.4.1.36 | Streptomyces venezuelae ATCC 10712 | F2RH01 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.36 | nickel affinity column chromatography and Superdex 200 gel filtration | Streptomyces venezuelae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.36 | ADP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.36 | ADP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae ATCC 10712 | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.36 | GDP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.36 | GDP-glucose + glucose 6-phosphate | the enzyme has a preference for GDP-glucose | Streptomyces venezuelae | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.36 | GDP-glucose + glucose 6-phosphate | - |
Streptomyces venezuelae ATCC 10712 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.36 | GDP-glucose + glucose 6-phosphate | the enzyme has a preference for GDP-glucose | Streptomyces venezuelae ATCC 10712 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.36 | homodimer | 2 * 51000, calculated from amino acid sequence | Streptomyces venezuelae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.36 | alpha,alpha-trehalose-phosphate synthase | - |
Streptomyces venezuelae |
2.4.1.36 | OtsA | - |
Streptomyces venezuelae |
2.4.1.36 | trehalose-6-phosphate synthase | - |
Streptomyces venezuelae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.36 | 26 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 29 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 123 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 139 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.36 | 29 | - |
glucose 6-phosphate | with 2.5 mM ADP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 67 | - |
ADP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 132 | - |
glucose 6-phosphate | with 0.5 mM GDP-glucose as cosubstrate, pH and temperature not specified in the publication | Streptomyces venezuelae | |
2.4.1.36 | 1143 | - |
GDP-glucose | pH and temperature not specified in the publication | Streptomyces venezuelae |