Literature summary extracted from

Lee, S.G.; Jez, J.M.
Conformational changes in the di-domain structure of Arabidopsis phosphoethanolamine methyltransferase leads to active-site formation (2017), J. Biol. Chem., 292, 21690-21702 .

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.1.1.103	structure in complex with S-adenosyl-L-methionine	Arabidopsis thaliana
2.1.1.103	structure in complex with S-adenosyl-L-methionine, to 1.5 A resolution	Arabidopsis thaliana

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.1.1.103	0.069	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.0897	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.093	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.156	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.201	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.212	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.215	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.249	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.278	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.284	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.308	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.408	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.507	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.577	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.653	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.1.103	Arabidopsis thaliana	Q944H0	-	-
2.1.1.103	Arabidopsis thaliana	Q9C6B9	-	-
2.1.1.103	Arabidopsis thaliana	Q9FR44	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.1.1.103	ethanolamine phosphate + S-adenosyl-L-methionine	-	Arabidopsis thaliana	N-methylethanolamine phosphate + S-adenosyl-L-homocysteine	-	?
2.1.1.103	N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine	-	Arabidopsis thaliana	choline phosphate + S-adenosyl-L-homocysteine	-	?
2.1.1.103	N-methylethanolamine phosphate + S-adenosyl-L-methionine	-	Arabidopsis thaliana	N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.1.1.103	?	x * 56100, calculated from sequence	Arabidopsis thaliana
2.1.1.103	?	x * 57800, calculated from sequence	Arabidopsis thaliana

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.1.1.103	0.03	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.04	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.04	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.06	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.09	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.13	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.14	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.17	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.21	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.21	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.21	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.22	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.27	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.27	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.27	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana

General Information

EC Number	General Information	Comment	Organism
2.1.1.103	physiological function	isoform NMT1 is essential for normal growth and salt tolerance. The NMT1 mutant displays a short root phenotype under normal growth conditions and hypersensitivity, as determined by root length, under salt-stress conditions	Arabidopsis thaliana

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.1.1.103	0.161	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.209	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.266	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.305	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.367	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.408	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.433	-	N,N-dimethylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.489	-	N-methylethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.658	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.767	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.866	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	0.897	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	1.006	-	S-adenosyl-L-methionine	cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	1.314	-	Ethanolamine phosphate	pH 8.0, temperature not specified in the publication	Arabidopsis thaliana
2.1.1.103	1.453	-	S-adenosyl-L-methionine	cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication	Arabidopsis thaliana

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Release 2023.1 (January 2023)