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Literature summary extracted from

  • Drees, S.; Li, C.; Prasetya, F.; Saleem, M.; Dreveny, I.; Williams, P.; Hennecke, U.; Emsley, J.; Fetzner, S.
    PqsBC, a condensing enzyme in the biosynthesis of the pseudomonas aeruginosa quinolone signal Crystal structure, inhibition, and reaction mechanism (2016), J. Biol. Chem., 291, 6610-6624 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.230 drug development the enzyme PqsBC is a potential drug target Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.230 the pqsB and pqsC genes of are cloned into plasmid pET28b and expresssed in Escherichia coli Rosetta2(DE3) Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.230 sitting drop vapor diffusion method, PqsBC(C129A) crystallized in space group P2(1)2(1)2(1) and the structure is determined to 2 A resolution, revealing that PqsB and PqsC have a pseudo-2-fold symmetry Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.230 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.230 octanoyl-CoA + (2-aminobenzoyl)acetate Pseudomonas aeruginosa ATCC 15692
-
 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.230 Pseudomonas aeruginosa Q9I4X2 AND Q9I4X1 Q9I4X2: 2-heptyl-4(1H)-quinolone synthase subunit PqsB, Q9I4X1: 2-heptyl-4(1H)-quinolone synthase subunit PqsC
-
2.3.1.230 Pseudomonas aeruginosa ATCC 15692 Q9I4X2 AND Q9I4X1 Q9I4X2: 2-heptyl-4(1H)-quinolone synthase subunit PqsB, Q9I4X1: 2-heptyl-4(1H)-quinolone synthase subunit PqsC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.230
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.230 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?
2.3.1.230 octanoyl-CoA + (2-aminobenzoyl)acetate
-
 Pseudomonas aeruginosa ATCC 15692 2-heptyl-4-quinolone + CoA + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.230 heterodimer
-
 Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
2.3.1.230 PqsBC
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.3.1.230 metabolism PqsBC, a key enzyme in the biosynthesis of the quorum-sensing signal molecules 2-heptyl-4(1H)-quinolone and Pseudomonas quinolone signal Pseudomonas aeruginosa