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Literature summary extracted from

  • Macdonald, P.J.; Francy, C.A.; Stepanyants, N.; Lehman, L.; Baglio, A.; Mears, J.A.; Qi, X.; Ramachandran, R.
    Distinct splice variants of dynamin-related protein 1 differentially utilize mitochondrial fission factor as an effector of cooperative GTPase activity (2016), J. Biol. Chem., 291, 493-507 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.5.5 cardiolipin stimulates Drp1, synergistically with mitochondrial fission factor Homo sapiens
3.6.5.5 cardiolipin stimulates Drp1, synergistically with mitochondrial fission factor Rattus norvegicus
3.6.5.5 Mff-proteoliposome lipid-stimulated GTPase activity Homo sapiens
3.6.5.5 Mff-proteoliposome lipid-stimulated GTPase activity Rattus norvegicus
3.6.5.5 mitochondrial fission factor Mff, stimulates Drp1 independently of, but synergistically with cardiolipin Homo sapiens
3.6.5.5 mitochondrial fission factor Mff, stimulates Drp1 independently of, but synergistically with cardiolipin Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.5 gene DNMTL1, recombinant expression of the splice variants of Drp1 in Escherichia coli strain BL21 Star (DE3) Homo sapiens
3.6.5.5 gene DNMTL1, recombinant expression of the splice variants of Drp1 in Escherichia coli strain BL21 Star (DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.5.5 additional information Drp1-A-only (712 aa, 699 aax0413-aa A-insert) and Drp1-B-only (742 aa, 755 aa, 13-aa A-insert) are introduced in the Drp1-long isozyme. A- and B-insert sequences both suppress Drp1 GTPase activity, with the B-insert exerting the most dominant inhibitory effect on these interactions Rattus norvegicus
3.6.5.5 additional information Drp1-A-only (712 aa, 699 aax0413-aa A-insert) and Drp1-B-only (742 aa, 755 aa, 13-aa A-insert) are introduced in the Drp1-short isozyme. A- and B-insert sequences both suppress Drp1 GTPase activity, with the B-insert exerting the most dominant inhibitory effect on these interactions Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.5.5 additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens
3.6.5.5 additional information
-
 additional information Michaelis-Menten kinetics Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.5.5 Mg2+ required Homo sapiens
3.6.5.5 Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.5 GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ?
3.6.5.5 GTP + H2O Rattus norvegicus
-
 GDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.5 Homo sapiens O00429
-
-
3.6.5.5 Rattus norvegicus O35303
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.5 GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ?
3.6.5.5 GTP + H2O
-
 Rattus norvegicus GDP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.5.5 More Drp1 isoforms exhibit differential oligomerization propensities in solution and distinct helical geometry on cardiolipin-containing membranes, analysis of Drp1-long, Drp1-A-only, and Drp1-B-only by gel filtration Rattus norvegicus
3.6.5.5 More Drp1 isoforms exhibit differential oligomerization propensities in solution and distinct helical geometry on cardiolipin-containing membranes, analysis of Drp1-short, Drp1-A-only, and Drp1-B-only by gel filtration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.5.5 DNM1L
-
 Homo sapiens
3.6.5.5 DNM1L
-
 Rattus norvegicus
3.6.5.5 Drp1-long
-
 Rattus norvegicus
3.6.5.5 Drp1-short isozyme 3 Homo sapiens
3.6.5.5 dynamin-1-like protein
-
 Rattus norvegicus
3.6.5.5 dynamin-related protein 1
-
 Homo sapiens
3.6.5.5 dynamin-related protein 1
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.5.5 37
-
 assay at Homo sapiens
3.6.5.5 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.5.5 7.5
-
 assay at Homo sapiens
3.6.5.5 7.5
-
 assay at Rattus norvegicus

General Information

EC Number General Information Comment Organism
3.6.5.5 additional information Drp1 isoforms exhibit differential GTPase activities, comparison of splice variants, overview. Human isozyme Drp1-short exhibits greater cooperative GTPase activities relative to rat Drp1-long Homo sapiens
3.6.5.5 additional information Drp1 isoforms exhibit differential GTPase activities, comparison of splice variants, overview. Human isozyme Drp1-short exhibits greater cooperative GTPase activities relative to rat Drp1-long Rattus norvegicus
3.6.5.5 physiological function distinct splice variants of dynamin-related protein 1 differentially utilize mitochondrial fission factor as an effector of cooperative GTPase activity Homo sapiens
3.6.5.5 physiological function distinct splice variants of dynamin-related protein 1 differentially utilize mitochondrial fission factor as an effector of cooperative GTPase activity Rattus norvegicus