EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.6 | gene SA1684, quantitative reverse transcription-PCR analysis, recombinant expression of C-terminally His-tagged SA1684 protein in Escherichia coli strain BL21(DE3)-RIPL | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.6 | D106A | site-directed mutagenesis, the mutant shows highly reduced nucleoside diphosphatase activity | Staphylococcus aureus |
3.6.1.6 | D123A/E124A | site-directed mutagenesis, the mutant shows highly reduced nucleoside diphosphatase activity | Staphylococcus aureus |
3.6.1.6 | additional information | introduction of the wild-type SA1684 gene restores the hemolysin production of the SA1684-deletion mutant, whereas none of the alanine-substituted SA1684 mutant genes restores the hemolysin production. Construction of a DELTASA1684 deletion mutant, which exhibits drastically decreased virulence in a silkworm (Bombyx mori) infection model, in which the LD50 against silkworm larvae is more than 10times that of the parent strain. The SA1684-deletion mutant also exhibits decreased exotoxin production and colony-spreading ability. Introduction of wild-type SA1684 to the SA1684-deletion mutant restores the hemolysin production, nuclease production, and the colony-spreading activity | Staphylococcus aureus |
3.6.1.6 | Y88A | site-directed mutagenesis, the mutant shows highly reduced nucleoside diphosphatase activity | Staphylococcus aureus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.6 | GDP | substrate inhibition at high concentration | Staphylococcus aureus | |
3.6.1.6 | GDPbetaS | a non-hydrolyzable analogue of GDP | Staphylococcus aureus | |
3.6.1.6 | GTPgammaS | a non-hydrolyzable analogue of GTP | Staphylococcus aureus | |
3.6.1.6 | UDP | substrate inhibition at high concentration | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.6 | additional information | - |
additional information | Michaelis-Menten kinetics | Staphylococcus aureus | |
3.6.1.6 | 0.0963 | - |
ADP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 0.102 | - |
GDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 0.203 | - |
TDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 0.221 | - |
UDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 0.303 | - |
CDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.6 | Co2+ | dependent on | Staphylococcus aureus | |
3.6.1.6 | Mn2+ | dependent on, activates at 0.5-12.5 mM | Staphylococcus aureus | |
3.6.1.6 | additional information | no or very poor activation by Cu2+, Ni2+, Zn2+, Mg2+, and Ca2+ at 0.5 mM | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.6 | GDP + H2O | Staphylococcus aureus | - |
GMP + phosphate | - |
? | |
3.6.1.6 | GDP + H2O | Staphylococcus aureus N315 | - |
GMP + phosphate | - |
? | |
3.6.1.6 | UDP + H2O | Staphylococcus aureus | - |
UMP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.6 | Staphylococcus aureus | Q7A4T2 | - |
- |
3.6.1.6 | Staphylococcus aureus N315 | Q7A4T2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.6 | recombinant C-terminally His-tagged SA1684 protein from Escherichia coli strain BL21(DE3)-RIPL by nickel affinity chromatography to over 90% purity | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.6 | ADP + H2O | high activity | Staphylococcus aureus | AMP + phosphate | - |
? | |
3.6.1.6 | ADP + H2O | high activity | Staphylococcus aureus N315 | AMP + phosphate | - |
? | |
3.6.1.6 | CDP + H2O | moderate activity | Staphylococcus aureus | CMP + phosphate | - |
? | |
3.6.1.6 | dADP + H2O | low activity | Staphylococcus aureus | dAMP + phosphate | - |
? | |
3.6.1.6 | dCDP + H2O | low activity | Staphylococcus aureus | dCMP + phosphate | - |
? | |
3.6.1.6 | dGDP + H2O | low activity | Staphylococcus aureus | dGMP + phosphate | - |
? | |
3.6.1.6 | GDP + H2O | - |
Staphylococcus aureus | GMP + phosphate | - |
? | |
3.6.1.6 | GDP + H2O | moderate activity | Staphylococcus aureus | GMP + phosphate | - |
? | |
3.6.1.6 | GDP + H2O | - |
Staphylococcus aureus N315 | GMP + phosphate | - |
? | |
3.6.1.6 | GDP + H2O | moderate activity | Staphylococcus aureus N315 | GMP + phosphate | - |
? | |
3.6.1.6 | IDP + H2O | low activity | Staphylococcus aureus | IMP + phosphate | - |
? | |
3.6.1.6 | additional information | purified SA1684 protein has Mn2+- or Co2+-dependent hydrolyzing activity against nucleoside diphosphates. The enzyme shows or poor activity with NTPS or NMPs, substrate specificity, overview | Staphylococcus aureus | ? | - |
- |
|
3.6.1.6 | additional information | purified SA1684 protein has Mn2+- or Co2+-dependent hydrolyzing activity against nucleoside diphosphates. The enzyme shows or poor activity with NTPS or NMPs, substrate specificity, overview | Staphylococcus aureus N315 | ? | - |
- |
|
3.6.1.6 | TDP + H2O | moderate activity | Staphylococcus aureus | TMP + phosphate | - |
? | |
3.6.1.6 | TDP + H2O | moderate activity | Staphylococcus aureus N315 | TMP + phosphate | - |
? | |
3.6.1.6 | UDP + H2O | - |
Staphylococcus aureus | UMP + phosphate | - |
? | |
3.6.1.6 | UDP + H2O | best substrate | Staphylococcus aureus | UMP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.6 | ? | x * 28000, recombinant His-tagged enzyme, SDS-PAGE | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.6 | Nucleoside diphosphatase | - |
Staphylococcus aureus |
3.6.1.6 | SA1684 | - |
Staphylococcus aureus |
3.6.1.6 | SA1684 protein | - |
Staphylococcus aureus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.6 | 26 | - |
assay at | Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.6 | 0.357 | - |
GDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 0.415 | - |
ADP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 0.68 | - |
TDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 0.763 | - |
CDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 1.388 | - |
UDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.6 | 7.5 | - |
assay at | Staphylococcus aureus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.6.1.6 | evolution | the SA1684 gene product carries theDUF402domain, which is found in RNA-binding proteins, and has amino acid sequence similarity with a nucleoside diphosphatase, Streptomyces coelicolor SC4828 protein | Staphylococcus aureus |
3.6.1.6 | malfunction | the SA1684-deletion mutant exhibits drastically decreased virulence in a silkworm (Bombyx mori) infection model, in which the LD50 against silkworm larvae is more than 10times that of the parent strain. The SA1684-deletion mutant also exhibits decreased exotoxin production and colony-spreading ability. Introduction of wild-type SA1684 to the SA1684-deletion mutant restores the hemolysin production, nuclease production, and the colony-spreading activity | Staphylococcus aureus |
3.6.1.6 | additional information | the amino acid residues Tyr88, Asp106, and Asp123/Glu124 of SA1684 protein are required for NDP hydrolysis and Staphylococcus aureus virulence | Staphylococcus aureus |
3.6.1.6 | physiological function | RNA sequence analysis reveals thatSA1684 is required for the expression of the virulence regulatory genes agr, sarZ, and sarX, as well as metabolic genes involved in glycolysis and fermentation pathways. These findings suggest that the nucleoside diphosphatase SA1684 links metabolic pathways and virulence gene expression and plays an important role in Staphylococcus aureus virulence in silkworms. Introduction of wild-type SA1684 to the SA1684-deletion mutant restores the hemolysin production, nuclease production, and the colony-spreading activity. Enzyme SA1684 is required for hemolysin production, nuclease production, and colony spreading | Staphylococcus aureus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.6 | 2.518 | - |
CDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 3.35 | - |
TDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 3.5 | - |
GDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 4.31 | - |
ADP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus | |
3.6.1.6 | 6.281 | - |
UDP | recombinant enzyme, pH 7.5, 26°C | Staphylococcus aureus |