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Literature summary extracted from

  • Lin, S.; McLennan, A.G.; Ying, K.; Wang, Z.; Gu, S.; Jin, H.; Wu, C.; Liu, W.; Yuan, Y.; Tang, R.; Xie, Y.; Mao, Y.
    Cloning, expression, and characterization of a human inosine triphosphate pyrophosphatase encoded by the itpa gene (2001), J. Biol. Chem., 276, 18695-18701 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.9
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.31
-
 dITP pH 8.5, 37°C Homo sapiens
3.6.1.9 0.51
-
 ITP pH 8.5, 37°C Homo sapiens
3.6.1.9 0.57
-
 xanthosine 5'-triphosphate pH 8.5, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.9 Mg2+ activity increases sharply up to about 10 mM MgCl2 and reaches a plateau between about 30 and 100 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.9 25000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Homo sapiens Q9BY32
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.9 adrenal gland
-
 Homo sapiens
-
3.6.1.9 heart
-
 Homo sapiens
-
3.6.1.9 liver
-
 Homo sapiens
-
3.6.1.9 thyroid gland
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 dITP + H2O at the optimal pH of 10.0, the recombinant enzyme hydrolyzes ITP, dITP, and xanthosine 5'-triphosphate to their respective monophosphates whereas activity with other nucleoside triphosphates is low. UTP, (d)CTP, TTP, and (d)ATP are hydrolyzed 10-100fold less than dITP. IDP and IMP are not hydrolyzed at all Homo sapiens dIMP + diphosphate
-
 ?
3.6.1.9 ITP + H2O at the optimal pH of 10.0, the recombinant enzyme hydrolyzes ITP, dITP, and xanthosine 5'-triphosphate to their respective monophosphates whereas activity with other nucleoside triphosphates is low. UTP, (d)CTP, TTP, and (d)ATP are hydrolyzed 10-100fold less than dITP. IDP and IMP are not hydrolyzed at all Homo sapiens IMP + diphosphate
-
 ?
3.6.1.9 xanthosine 5'-triphosphate + H2O at the optimal pH of 10.0, the recombinant enzyme hydrolyzes ITP, dITP, and xanthosine 5'-triphosphate to their respective monophosphates whereas activity with other nucleoside triphosphates is low. UTP, (d)CTP, TTP, and (d)ATP are hydrolyzed 10-100fold less than dITP. IDP and IMP are not hydrolyzed at all Homo sapiens xanthosine 5'-monophosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.9 ? x * 25000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.6.1.9 inosine triphosphate pyrophosphatase
-
 Homo sapiens
3.6.1.9 ITPase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.9 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.9 360
-
 dITP pH 8.5, 37°C Homo sapiens
3.6.1.9 580
-
 ITP pH 8.5, 37°C Homo sapiens
3.6.1.9 640
-
 xanthosine 5'-triphosphate pH 8.5, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 10
-
 in glycine buffer Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.9 1120
-
 xanthosine 5'-triphosphate pH 8.5, 37°C Homo sapiens
3.6.1.9 1140
-
 ITP pH 8.5, 37°C Homo sapiens
3.6.1.9 1160
-
 dITP pH 8.5, 37°C Homo sapiens