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Literature summary extracted from

  • van der Sluis, R.; Ungerer, V.; Nortje, C.; A van Dijk, A.; Erasmus, E.
    New insights into the catalytic mechanism of human glycine N-acyltransferase (2017), J. Biochem. Mol. Toxicol., 31, e21963 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.13 analysis determination of kinetic parameters of recombinant human enzyme using the traditional colorimetric method and a HPLC-ESI-MS/MSmethod Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.13 expression in Escherichia coli Homo sapiens
2.3.1.71 expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.13 2.189
-
 glycine K0.5 value at 0.02 mM benzoyl-CoA, pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.13 2.326
-
 glycine K0.5 value at 0.04 mM benzoyl-CoA, pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.13 2.956
-
 glycine K0.5 value at 0.08 mM benzoyl-CoA, pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.13 30.42
-
 benzoyl-CoA K0.5 value at 2.5 mM gylcine, pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.13 108.3
-
 benzoyl-CoA K0.5 value at 5 mM gylcine, pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.13 305
-
 benzoyl-CoA K0.5 value at 20 mM gylcine, pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.71 0.049
-
 benzoyl-CoA purified recombinant S156 haplotype, apparent value, random bi-bi (sequential) mechanism, at pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.71 0.053
-
 benzoyl-CoA purified recombinant S156 haplotype, apparent value, ping-pong bi-bi (non-sequential) mechanism, at pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.71 0.06
-
 benzoyl-CoA purified recombinant S156 haplotype, apparent value, ordered bi-bi (sequential) mechanism, at pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.71 5
-
 glycine purified recombinant S156 haplotype, apparent value, ordered bi-bi (sequential) mechanism, at pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.71 20
-
 glycine purified recombinant S156 haplotype, apparent value, random bi-bi (sequential) mechanism, at pH 8.0, temperature not specified in the publication Homo sapiens
2.3.1.71 21
-
 glycine purified recombinant S156 haplotype, apparent value, ping-pong bi-bi (non-sequential) mechanism, at pH 8.0, temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.71 benzoyl-CoA + glycine Homo sapiens
-
 CoA + hippurate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.13 Homo sapiens Q6IB77
-
-
2.3.1.71 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.71 nickel-affinity column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.71 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.13 benzoyl-CoA + glycine
-
 Homo sapiens CoA + N-benzoylglycine
-
 ?
2.3.1.13 additional information GLYAT exhibits mechanistic kinetic cooperativity Homo sapiens ?
-
-
2.3.1.71 benzoyl-CoA + glycine
-
 Homo sapiens CoA + hippurate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.13 GlyAT
-
 Homo sapiens
2.3.1.71 GlyAT
-
 Homo sapiens
2.3.1.71 glycine N-acyltransferase
-
 Homo sapiens