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Literature summary extracted from

  • Fu, H.; Goettge, M.; Metcalf, W.
    Biochemical characterization of the methylmercaptopropionate Cob(I)alamin methyltransferase from Methanosarcina acetivorans (2019), J. Bacteriol., 201, e00130-19 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.90 0.0122
-
 methylcob(III)alamin at pH 7.2, temperature not specified in the publication Methanosarcina acetivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.90 Zn2+ 0.1 mM used in assay conditions Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.90 methanol + cob(I)alamin Methanosarcina acetivorans methylation of free cob(I)alamin by methylmercaptopropionate, which is the physiologically relevant direction, is observed only in the presence of very high levels of methylmercaptopropionate methylcob(III)alamin + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.90 Methanosarcina acetivorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.90 Strep-Tactin resin column chromatography and Superdex S200 gel filtration Methanosarcina acetivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.90 mercaptopropionate + methylcob(III)alamin
-
 Methanosarcina acetivorans ?
-
 ?
2.1.1.90 methanol + cob(I)alamin methylation of free cob(I)alamin by methylmercaptopropionate, which is the physiologically relevant direction, is observed only in the presence of very high levels of methylmercaptopropionate Methanosarcina acetivorans methylcob(III)alamin + H2O
-
 ?
2.1.1.90 additional information coenzyme M does not serve as a methyl acceptor. Methylation of cob(I)alamin with methanol, dimethylsulfide, dimethylamine, and methyl-CoM is not observed, even at high substrate concentrations Methanosarcina acetivorans ?
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-

Synonyms

EC Number Synonyms Comment Organism
2.1.1.90 Cob(I)alamin methyltransferase
-
 Methanosarcina acetivorans
2.1.1.90 MMPA:cob(I)alamin methyltransferase
-
 Methanosarcina acetivorans
2.1.1.90 MtpA
-
 Methanosarcina acetivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.90 0.315
-
 methylcob(III)alamin at pH 7.2, temperature not specified in the publication Methanosarcina acetivorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.90 26
-
 methylcob(III)alamin at pH 7.2, temperature not specified in the publication Methanosarcina acetivorans