EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | expressed in Escherichia coli BL21(DE3)-pLysS cells | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.23 | mutant enzymes R140K/H145W and G143STOP, hanging drop vapor diffusion method, using 0.1 M Tris/HCl pH 7.5, 1.5 M ammonium sulfate, and 12.5% (v/v) glycerol | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.23 | G143STOP | the mutant shows severely reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
3.6.1.23 | H145W | the mutant shows strongly reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
3.6.1.23 | R40K/H145W | the mutant shows severely reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
3.6.1.23 | T138STOP | the mutant shows severely reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.0011 | - |
dUTP | mutant enzyme R140K/H145W, at pH 7.5 and 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 0.0013 | - |
dUTP | mutant enzyme H145W, at pH 7.5 and 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 0.0014 | - |
dUTP | wild type enzyme, at pH 7.5 and 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 0.0067 | - |
dUTP | mutant enzyme T138STOP, at pH 7.5 and 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 0.0082 | - |
dUTP | mutant enzyme G143STOP, at pH 7.5 and 20°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | Mycobacterium tuberculosis | - |
dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | Mycobacterium tuberculosis H37Rv | - |
dUMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Mycobacterium tuberculosis | P9WNS5 | - |
- |
3.6.1.23 | Mycobacterium tuberculosis H37Rv | P9WNS5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.23 | Ni-NTA column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | - |
Mycobacterium tuberculosis | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Mycobacterium tuberculosis H37Rv | dUMP + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.23 | ? | x * 17836, electrospray ionization mass spectrometry | Mycobacterium tuberculosis |
3.6.1.23 | ? | x * 17966, calculated from amino acid sequence | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.23 | DUT | - |
Mycobacterium tuberculosis |
3.6.1.23 | dUTPase | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.00089 | - |
dUTP | mutant enzyme G143STOP, at pH 7.5 and 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 0.0035 | - |
dUTP | mutant enzyme T138STOP, at pH 7.5 and 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 0.0045 | - |
dUTP | mutant enzyme R140K/H145W, at pH 7.5 and 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 0.7 | - |
dUTP | mutant enzyme H145W, at pH 7.5 and 20°C | Mycobacterium tuberculosis | |
3.6.1.23 | 3 | - |
dUTP | wild type enzyme, at pH 7.5 and 20°C | Mycobacterium tuberculosis |