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Literature summary extracted from

  • Nagy, G.N.; Suardiaz, R.; Lopata, A.; Ozohanics, O.; Vekey, K.; Brooks, B.R.; Leveles, I.; Toth, J.; Vertessy, B.G.; Rosta, E.
    Structural characterization of arginine fingers identification of an arginine finger for the pyrophosphatase dUTPases (2016), J. Am. Chem. Soc., 138, 15035-15045 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli BL21(DE3)-pLysS cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.23 mutant enzymes R140K/H145W and G143STOP, hanging drop vapor diffusion method, using 0.1 M Tris/HCl pH 7.5, 1.5 M ammonium sulfate, and 12.5% (v/v) glycerol Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 G143STOP the mutant shows severely reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
3.6.1.23 H145W the mutant shows strongly reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
3.6.1.23 R40K/H145W the mutant shows severely reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
3.6.1.23 T138STOP the mutant shows severely reduced activity compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0011
-
dUTP mutant enzyme R140K/H145W, at pH 7.5 and 20°C Mycobacterium tuberculosis
3.6.1.23 0.0013
-
dUTP mutant enzyme H145W, at pH 7.5 and 20°C Mycobacterium tuberculosis
3.6.1.23 0.0014
-
dUTP wild type enzyme, at pH 7.5 and 20°C Mycobacterium tuberculosis
3.6.1.23 0.0067
-
dUTP mutant enzyme T138STOP, at pH 7.5 and 20°C Mycobacterium tuberculosis
3.6.1.23 0.0082
-
dUTP mutant enzyme G143STOP, at pH 7.5 and 20°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Mycobacterium tuberculosis
-
dUMP + diphosphate
-
?
3.6.1.23 dUTP + H2O Mycobacterium tuberculosis H37Rv
-
dUMP + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Mycobacterium tuberculosis P9WNS5
-
-
3.6.1.23 Mycobacterium tuberculosis H37Rv P9WNS5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
Mycobacterium tuberculosis dUMP + diphosphate
-
?
3.6.1.23 dUTP + H2O
-
Mycobacterium tuberculosis H37Rv dUMP + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 ? x * 17836, electrospray ionization mass spectrometry Mycobacterium tuberculosis
3.6.1.23 ? x * 17966, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 DUT
-
Mycobacterium tuberculosis
3.6.1.23 dUTPase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 0.00089
-
dUTP mutant enzyme G143STOP, at pH 7.5 and 20°C Mycobacterium tuberculosis
3.6.1.23 0.0035
-
dUTP mutant enzyme T138STOP, at pH 7.5 and 20°C Mycobacterium tuberculosis
3.6.1.23 0.0045
-
dUTP mutant enzyme R140K/H145W, at pH 7.5 and 20°C Mycobacterium tuberculosis
3.6.1.23 0.7
-
dUTP mutant enzyme H145W, at pH 7.5 and 20°C Mycobacterium tuberculosis
3.6.1.23 3
-
dUTP wild type enzyme, at pH 7.5 and 20°C Mycobacterium tuberculosis