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Literature summary extracted from

  • Daughtry, K.; Xiao, Y.; Stoner-Ma, D.; Cho, E.; Orville, A.; Liu, P.; Allen, K.
    Quaternary ammonium oxidative demethylation X-ray crystallographic, resonance Raman, and UV-visible spectroscopic analysis of a Rieske-type demethylase (2012), J. Am. Chem. Soc., 134, 2823-2834 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.247 expressed in Escherichia coli BL21(DE3) cells Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.247 in complex with stachydrine, hanging drop vapor diffusion method, using 15% (w/v) poly(ethylene glycol) 3350, 20% (v/v) glycerol, 100 mM succinic acid buffer, pH 7.0, and 10 mM hexamine cobalt at 22°C Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.247 stachydrine + NADH + H+ + O2 Sinorhizobium meliloti
-
 N-methyl-L-proline + formaldehyde + NAD+ + H2O
-
 ?
1.14.13.247 stachydrine + NADPH + H+ + O2 Sinorhizobium meliloti
-
 N-methyl-L-proline + formaldehyde + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.247 Sinorhizobium meliloti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.247 Strep-Tactin resin column chromatography Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.247 additional information no activity with N,N-dimethyl-glycine, N-methyl-glycine, and N-methyl-L-proline Sinorhizobium meliloti ?
-
-
1.14.13.247 stachydrine + NADH + H+ + O2
-
 Sinorhizobium meliloti N-methyl-L-proline + formaldehyde + NAD+ + H2O
-
 ?
1.14.13.247 stachydrine + NADPH + H+ + O2
-
 Sinorhizobium meliloti N-methyl-L-proline + formaldehyde + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.247 stachydrine demethylase
-
 Sinorhizobium meliloti
1.14.13.247 stc2
-
 Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.247 additional information non-heme enzyme Sinorhizobium meliloti
1.14.13.247 NADH
-
 Sinorhizobium meliloti
1.14.13.247 NADPH
-
 Sinorhizobium meliloti
1.14.13.247 [2Fe-2S]-center
-
 Sinorhizobium meliloti