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Literature summary extracted from

  • Zhang, L.; Gao, Y.; Liu, X.; Guo, F.; Ma, C.; Liang, J.; Feng, X.; Li, C.
    Mining of sucrose synthases from Glycyrrhiza uralensis and their application in the construction of an efficient UDP-recycling system (2019), J. Agric. Food Chem., 67, 11694-11702 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.13 expressed in Escherichia coli BL21(DE3) cells Glycyrrhiza uralensis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.13 SUS1-DELTA9 the kcat/Km of the mutant toward sucrose isdecreased by 28.4% compared to that of wild type isoform SUS1, while the kcat/Km of the mutant toward UDP is increased by 1.6% compared to that of SUS1 Glycyrrhiza uralensis
2.4.1.13 SUS2-DELTA9 the kcat/Km of the mutant toward UDP is increased by 3.8fold compared to that of wild type isoform SUS2, while the kcat/Km of the mutant toward sucrose is decreased by 35.7% compared to that of SUS2 Glycyrrhiza uralensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 0.3
-
UDP mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 0.59
-
UDP mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 0.74
-
UDP wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 0.89
-
UDP wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 5.41
-
sucrose wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 9.61
-
sucrose mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 24.47
-
sucrose wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 43.8
-
sucrose mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.13 NDP-alpha-D-glucose + D-fructose Glycyrrhiza uralensis
-
NDP + sucrose
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Glycyrrhiza uralensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13 Ni-NTA column chromatography Glycyrrhiza uralensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 NDP-alpha-D-glucose + D-fructose
-
Glycyrrhiza uralensis NDP + sucrose
-
r
2.4.1.13 UDP + sucrose
-
Glycyrrhiza uralensis UDP-alpha-D-glucose + D-fructose
-
r

Subunits

EC Number Subunits Comment Organism
2.4.1.13 ? x * 90000, SDS-PAGE Glycyrrhiza uralensis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.13 SUS1 isoform Glycyrrhiza uralensis
2.4.1.13 SUS2 isoform Glycyrrhiza uralensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.13 50
-
-
Glycyrrhiza uralensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.13 30 60 isoform SUS1 shows about 40% activity at 30°C, about 68% activity at 40°C, about 80% activity at 45°C, 100% activity at 50°C, about 85% activity at 55°C, and about 35% activity at 60°C. Isoform SUS2 shows about 50% activity at 30°C, about 60% activity at 40°C, about 80% activity at 45°C, 100% activity at 50°C, about 47% activity at 55°C, and about 30% activity at 60°C Glycyrrhiza uralensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.13 50
-
thermostability of wild type isoforms SUS1 and SUS2 is poor at 50°C. Activity loss occurs rapidly with increasing incubation time, and the half-lives are 50 and 25 min for SUS1 and SUS2, respectively. Mutant enzyme SUS1-DELTA9 shows a half-life increase of 2fold. The residual activity is 50% after 100 min, while the wild type activity remains less than 20% of the original activity. The half-life of the variant SUS2-DELTA9 also increases 2fold Glycyrrhiza uralensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.13 50
-
UDP mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 51.2
-
sucrose wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 62
-
UDP wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 74.8
-
UDP wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 76.8
-
sucrose mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 87.1
-
sucrose wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 99.6
-
sucrose mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 121.7
-
UDP mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 5.5
-
-
Glycyrrhiza uralensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.13 5 8 isoform SUS1 shows about 60% activity at pH 5.0, 100% activity at pH 5.5, about 90% activity at pH 6.5, about 40% activity at pH 7.5, and about 33% activity at pH 8.0. Isoform SUS2 shows about 50% activity at pH 5.0, 100% activity at pH 5.5, about 95% activity at pH 6.5, about 90% activity at pH 7.5, and about 70% activity at pH 8.0 Glycyrrhiza uralensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.13 2.29
-
sucrose mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 3.56
-
sucrose wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 8.01
-
sucrose mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 11.18
-
sucrose wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 84.3
-
UDP wild type enzyme isoform SUS2, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 84.42
-
UDP wild type enzyme isoform SUS1, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 85.75
-
UDP mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis
2.4.1.13 407.83
-
UDP mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C Glycyrrhiza uralensis